BACKGROUND/AIMS:The urinary concentration of the monocyte chemoattractant protein-1 (uMCP-1) chemokine is increased in several proteinuric and/or inflammatory renal diseases. In the present study, we evaluated the association between uMCP-1 and renal function, proteinuria, glomerular and interstitial macrophage infiltration, and renal fibrosis in patients with primary and secondary glomerulopathies diagnosed by renal biopsy. METHODS:Thirty-seven patients aged 32.6 +/- 7.7 years were studied. uMCP-1 was determined by ELISA. Renal macrophage expression (CD68 positive cells) is reported as number of macrophages/10(4) microm2 of the cortical tubulointerstitial (TI) area or of glomerular capillary tuft area. Cortical interstitial fibrosis was quantitated by PicroSirius red staining under polarized light by a computerized manner. RESULTS:The uMCP-1 ratio (pg/ml/urinary creatinine mg/ml) was positively correlated (Spearman coefficient) with proteinuria (r = 0.4629; p < 0.005) and number of macrophages in the cortical TI area (r = 0.64; p = 0.0005), and negatively correlated with creatinine clearance (r = -0.4877; p < 0.001). The uMCP-1 ratio was not significantly correlated with number of macrophages/glomerular capillary tuft area (r = 0.27; p = 0.19) or with percent cortical interstitial fibrosis (r = 0.08; p = 0.62). CONCLUSIONS:The uMCP-1 excretion is a biomarker of the inflammatory activity of the TI area, and does not reflect chronic interstitial damage.

译文

背景/目的:在几种蛋白尿和/或炎性肾脏疾病中,单核细胞趋化蛋白-1(uMCP-1)趋化因子的尿液浓度增加。在本研究中,我们评估了通过肾活检诊断为原发性和继发性肾小球病变的患者中uMCP-1与肾功能,蛋白尿,肾小球和间质巨噬细胞浸润以及肾纤维化之间的关系。
方法:对37名年龄在32.6 /-7.7岁的患者进行了研究。通过ELISA确定uMCP-1。肾巨噬细胞表达(CD68阳性细胞)报告为皮质肾小管间质(TI)区域或肾小球毛细血管簇区域的巨噬细胞数/ 10(4)microm2。皮层间质纤维化通过计算机化方式在偏光下通过PicroSirius红染色进行定量。
结果:uMCP-1比值(pg / ml /尿肌酐mg / ml)与蛋白尿(r = 0.4629; p <0.005)和皮质TI区域巨噬细胞数量(r = 0.64; n = 0.64)呈正相关(Spearman系数)。 p = 0.0005),与肌酐清除率呈负相关(r = -0.4877; p <0.001)。 uMCP-1比率与巨噬细胞/肾小球毛细血管簇面积(r = 0.27; p = 0.19)或皮质间质纤维化百分比(r = 0.08; p = 0.62)没有显着相关性。
结论:uMCP-1排泄是TI区炎症活动的生物标志物,不能反映慢性间质损伤。

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