Leishmania major-specific T cell lines were derived from mice sensitized to the parasite. The cells were of the CD4+ T cell lineage and, upon adoptive transfer, were found to be capable of inducing parasite-specific delayed-type hypersensitivity. Adoptive transfer of these L. major-specific T cells to syngeneic recipients which were either normal, T cell deficient or B cell and antibody deficient led to exacerbation of infection upon subsequent challenge with L. major. This suggested that host T cells, B cells and antibody were not required for the L. major-specific T cells to exert their exacerbative effect on the course of cutaneous leishmaniasis. Additional studies revealed that the adoptive transfer of graded doses of these L. major-specific T cells always resulted in exacerbation of infection. Study of the localization pattern of the cells following transfer showed that they migrate preferentially to the site of the lesions. Furthermore, although the induction phase of this phenomenon was immunologically specific, its effector phase was not. Finally, T cell clones were derived from the L. major-specific T cell lines. The T cell clones were phenotypically and functionally identical to the T cell lines from which they were derived. Adoptive transfer of these parasite-specific T cell clones to normal syngeneic recipients induced an exacerbated course of infection with L. major. Interestingly, when these cloned T cells were specifically activated in vitro, the cells produced interleukin 2 and interferon-gamma, but no interleukin 4, indicating that they belong to the murine Th1 subset of CD4+ T cells.

译文

:利什曼原虫主要特异性T细胞系来源于对寄生虫敏感的小鼠。这些细胞属于CD4 T细胞谱系,经过继转移后,能够诱导寄生虫特异性迟发型超敏反应。将这些大肠埃希氏菌特异性T细胞过继转移至正常的T细胞缺陷或B细胞和抗体缺乏的同基因受体,在随后用大肠埃希氏菌攻击后导致感染加重。这表明主要的L.特异性T细胞在皮肤利什曼病病程中发挥加重作用并不需要宿主T细胞,B细胞和抗体。进一步的研究表明,这些主要乳酸链球菌T细胞分级给药的过继转移总是导致感染加重。转移后细胞定位模式的研究表明,它们优先迁移至病变部位。此外,尽管该现象的诱导期在免疫学上是特异性的,但其效应期不是。最后,T细胞克隆衍生自L. major-特异性T细胞系。 T细胞克隆在表型和功能上与它们衍生的T细胞系相同。这些寄生虫特异的T细胞克隆过继转移至正常的同基因受体,导致大肠埃希氏菌感染的病程加剧。有趣的是,当这些克隆的T细胞在体外被特异性激活时,这些细胞产生白介素2和干扰素-γ,但不产生白介素4,表明它们属于CD4 T细胞的鼠Th1子集。

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