PURPOSE:To identify the relationship between epidermal growth factor (EGF) concentration in human tears and clinical tear-flow parameters and how these vary with age and gender.

METHODS:Tear samples were collected with minimal stimulation from 68 healthy and asymptomatic adults (33 men, 35 women), aged 21-88 years. EGF concentrations were determined by sandwich enzyme-linked immunosorbent assay (ELISA) in 65 cases. Schirmer tests were performed without anesthesia, and the clearance of fluorescein from the tear film assessed. The Tear Function Index (TFI) was calculated from these values.

RESULTS:There were approximately equal numbers of male and female subjects with a similar age distribution for each gender (48 +/- 3 and 51 +/- 3 years, mean +/- SEM, respectively). Ninety percent of tear EGF concentrations were between 0.75 and 7.1 ng/ml. Tear EGF level correlated significantly with Schirmer I value, but not with age. Schirmer I value correlated with tear clearance [LN(TCR)] but not with age. Tear EGF concentrations were significantly higher for men (3.4 +/- 0.3 ng/ml) than for women (2.4 +/- 0.3 ng/ml; p = 0.043).

CONCLUSIONS:EGF concentrations is tear samples from normal humans were found to correlate with gender and Schirmer I value but not with tear clearance.

译文

目的 : 确定人眼泪中表皮生长因子 (EGF) 浓度与临床泪液流量参数之间的关系,以及这些参数随年龄和性别的变化。
方法 : 在最小的刺激下,从68名健康和无症状的成年人 (33名男性,35名女性) 收集了眼泪样本,年龄在21-88岁之间。用夹心酶联免疫吸附法 (ELISA) 测定65例患者的EGF浓度。在没有麻醉的情况下进行Schirmer测试,并评估了泪膜中荧光素的清除率。泪液功能指数 (TFI) 是根据这些值计算得出的。
结果 : 每个性别年龄分布相似的男性和女性受试者数量大致相等 (48/- 3岁和51/- 3岁,分别为平均值 +/- SEM)。90% 的泪液EGF浓度在0.75至7.1 ng/ml之间。泪液EGF水平与Schirmer I值显着相关,但与年龄无关。Schirmer I值与泪液清除率 [LN(TCR)] 相关,但与年龄无关。男性的泪液EGF浓度 (3.4/- 0.3 ng/ml) 显着高于女性 (2.4/- 0.3 ng/ml; p = 0.043)。
结论 :EGF浓度是正常人的泪液样本与性别和Schirmer I值相关,但与泪液清除率无关。

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