Adventitious roots of Echinacea purpurea were cultured in airlift bioreactors (20 l, 500 l balloon-type, bubble bioreactors and 1,000 l drum-type bubble bioreactor) using Murashige and Skoog (MS) medium with 2 mg indole butyric acid l(-1) and 50 g sucrose l(-1) for the production of chichoric acid, chlorogenic acid and caftaric acid. In the 20 l bioreactor (containing 14 l MS medium) a maximum yield of 11 g dry biomass l(-1) was achieved after 60 days. However, the amount of total phenolics (57 mg g(-1) DW), flavonoids (34 mg g(-1) DW) and caffeic acid derivatives (38 mg g(-1) DW) were highest after 50 days. Based on these studies, pilot-scale cultures were established and 3.6 kg and 5.1 kg dry biomass were achieved in the 500 l and 1,000 l bioreactors, respectively. The accumulation of 5 mg chlorogenic acid g(-1) DW, 22 mg chichoric acid g(-1) DW and 4 mg caftaric acids g(-1) DW were achieved with adventitious roots grown in 1,000 l bioreactors.

译文

:紫锥菊的次生根在装有2 mg吲哚丁酸l(-1)的Murashige和Skoog(MS)培养基中于气举生物反应器(20 l,500 l气球型,气泡生物反应器和1,000 l鼓型气泡生物反应器)中培养。 )和50克蔗糖l(-1)用于生产手性酸,绿原酸和异戊酸。 60天后,在20升生物反应器(包含14升MS培养基)中,最大产量为11克干燥生物质l(-1)。但是,总酚含量(57 mg g(-1)DW),类黄酮(34 mg g(-1)DW)和咖啡酸衍生物(38 mg g(-1)DW)的量在50天后最高。基于这些研究,建立了中试规模的培养,分别在500 l和1,000 l生物反应器中实现了3.6 kg和5.1 kg干燥生物量。 5 mg绿原酸g(-1)DW,22 mg chichoric acid g(-1)DW和4 mg caftaric acid g(-1)DW的积累是通过在1,000升生物反应器中生长的不定根实现的。

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