This study was aimed to investigate the dermal irritant test and the central analgesic effects of topical application of seven different formulations (A-G) of analgesic gel prepared from locally available raw material in animal model. The results of dermal irritant test revealed that no animal showed any kind of toxic effect i.e. redness, irritation, itching, inflammation, skin infection or any other injurious effects. All animals remained healthy, active, alert showing normal behavior and no mortality was observed during the claimed period. The analgesic activity was performed by tail flick test. The analgesic activity against tail flick test revealed that all samples of test gel had analgesic effect at 15, 30 and 60 minutes after sample application but sample D had highest analgesic effects (193%) followed by sample E (155%), sample C (122%), sample G (85%), sample B (84%),sample F (81%) while sample A exhibited (73%) analgesic activity. Wintogeno was run as standard drug and it showed 168% analgesic activity.

译文

本研究旨在研究动物模型中由局部可用原料制备的七种不同配方 (A-G) 的镇痛凝胶的皮肤刺激性试验和局部应用的中枢镇痛作用。皮肤刺激性测试的结果表明,没有动物表现出任何毒性作用,即发红,刺激,瘙痒,炎症,皮肤感染或任何其他有害作用。所有动物都保持健康,活跃,警觉,表现出正常行为,在要求保护的期间内未观察到死亡率。镇痛活性通过甩尾试验进行。对甩尾试验的镇痛活性表明,试验凝胶的所有样品在样品施用后15、30和60分钟均具有镇痛作用,但样品D具有最高的镇痛作用 (193%),其次是样品E (155%),样品C (122%),样品G (85%),样品B (84%),样品F (81%),而样品A表现出 (73%) 镇痛活性。Wintogeno作为标准药物运行,它显示出168% 的镇痛活性。

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