We have reported recently that Interleukin-12 (IL-12) released from poly-N-acetyl glucosamine gel matrix (F2 gel/IL-12) is more effective than free IL-12 to enhance vaccination of mice with Schistosoma soluble worm antigen preparation. The aim of this study is to evaluate the effect of F2 gel/IL-12 on the inflammatory responses in mice undergoing schistosomiasis infection in absence of vaccination. To achieve this, mice undergoing Schistosoma mansoni infection or cured from this infection, after treatment with praziquantil (PZQ), were treated with subcutaneous injection of IL-12 for 3 consecutive days or once with F2 gel loaded with IL-12 (F2 gel/IL-12). The treatment was started on day 35 days after infection. For infection, mice were infected with 100 cercariae of S. mansoni using tail immersion method. We found that treatment with F2 gel/IL-12 induced significant decreases in the egg burden with a moderate reduction in the size of granuloma and decrease in the cellular granulomatous reaction in the lung as compared to infected mice treated with IL-12. These effects of F2 gel/IL-12 were more pronounced in infected mice previously treated with the anti-schistosomal drug PZQ. The total numbers of white blood cells in all treated mice showed similar profile. Treatment with IL-12 or F2 gel/IL-12, however, showed significant reduction in the number of mononuclear cells when compared with non-treated infected mice. In conclusion, this study showed the ability of IL-12 released from F2 gel to lower the inflammatory response to Schistosoma infection even in absence of vaccination.

译文

我们最近报道了从聚-N-乙酰氨基葡萄糖凝胶基质 (F2凝胶/IL-12) 释放的Interleukin-12 (IL-12) 比游离IL-12更有效地增强用血吸虫可溶性蠕虫抗原制剂接种小鼠的疫苗。这项研究的目的是评估F2凝胶/IL-12对在没有疫苗接种的情况下接受血吸虫病感染的小鼠炎症反应的影响。为了实现这一点,接受曼氏血吸虫感染或从这种感染中治愈的小鼠,在用吡喹酮 (PZQ) 治疗后,连续3天皮下注射IL-12,或用装有IL-12的F2凝胶 (F2凝胶/IL-12) 治疗一次。在感染后第35天开始治疗。对于感染,使用尾浸法用曼氏杆菌的100尾c感染小鼠。我们发现,与用IL-12治疗的感染小鼠相比,用F2凝胶/IL-12治疗可导致卵负荷显着降低,肉芽肿大小适度减少,肺中细胞肉芽肿反应减少。F2凝胶/IL-12的这些作用在先前用抗血吸虫药物PZQ治疗的感染小鼠中更为明显。在所有处理过的小鼠中,白细胞总数显示出相似的特征。然而,与未处理的感染小鼠相比,用IL-12或F2凝胶/IL-12处理显示单核细胞数量显著减少。总之,这项研究表明,即使在没有接种疫苗的情况下,从F2凝胶中释放的IL-12也能够降低对血吸虫感染的炎症反应。

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