Piwi (P-element induced wimpy testis) is an important gene involved in stem cell maintenance and gametogenesis in vertebrates. However, in most invertebrates, especially mollusks, the function of Piwi during gametogenesis remains largely unclear. To further understand the function of Piwi during gametogenesis, full-length cDNA of Piwi1 from scallop Chlamys farreri (Cf-Piwi1) was characterized, which consisted of a 2,637 bp open reading frame encoding an 878-amino acid protein. Cf-Piwi1 mRNA was mainly localized in the spermatogonia, spermatocytes, oogonia, oocytes of early development and intra-gonadal somatic cells. Additionally, the knockdown of Cf-Piwi1 by injection of Cf-Piwi1-dsRNA (double-stranded RNA) into scallop adductor led to a loss of germ cells in C. farreri gonads. Apoptosis was observed mainly in spermatocytes and oocytes of early development, as well as in a small number of spermatogonia and oogonia. Our findings indicate that Cf-Piwi1 is essential for gametogenesis in the scallop C. farreri.

译文

:Piwi(P元素诱导的w弱睾丸)是一个重要的基因,参与脊椎动物的干细胞维持和配子发生。但是,在大多数无脊椎动物中,尤其是软体动物中,Piwi在配子发生过程中的功能仍然不清楚。为了进一步了解Piwi在配子发生过程中的功能,对扇贝衣原体(Cf-Piwi1)的Piwi1全长cDNA(Cf-Piwi1)进行了表征,该cDNA包含一个2,637 bp的开放阅读框,编码878个氨基酸。 Cf-Piwi1 mRNA主要位于精原细胞,精细胞,卵子,早期发育的卵母细胞和性腺内的体细胞中。此外,通过将Cf-Piwi1-dsRNA(双链RNA)注入扇贝加合物中来敲低Cf-Piwi1导致法氏梭菌性腺中生殖细胞的损失。细胞凋亡主要在早期发育的精母细胞和卵母细胞中观察到,在少数精原细胞和卵母细胞中也观察到。我们的发现表明,Cf-Piwi1对于扇贝C. farreri的配子发生至关重要。

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