BACKGROUND:We examined loss of heterozygosity (LOH) of the P53 gene in bladder cancer, and investigated the role of the P53 gene on malignant progression of papillary tumors. In addition, the clonality of recurrent bladder cancer was examined. METHODS:LOH of the P53 gene was analyzed in 67 bladder cancers from 47 patients. DNA was extracted from formalin-fixed, paraffin-embedded tissues, amplified by the polymerase chain reaction (PCR) at 3 polymorphic loci in the P53 gene, and analyzed with nonradioisotopic single-strand conformation polymorphism (Non-RI SSCP) analysis. RESULTS:Out of 40 informative samples, LOH was detected in 13 samples, containing 4 of 7 in grade 3 (57%), 9 of 23 in grade 2 (39%), and none of 10 in grade 1 (10%). Statistical significance was observed between the LOH in grades 1 and 2, and in grades 1 and 3. An analysis of 5 cases showing malignant progression revealed that 3 (60%) showed an LOH in the primary tumor, and 2 showed LOH in recurrent tumors, in contrast to LOH found in 3 cases of 19 (16%) not showing malignant progression. Four cases with metachronous recurrence exhibited LOH; 2 at recurrent tumors, 1 only at the initial tumor, and 1 at both tumors. CONCLUSIONS:The alterations of the P53 gene were considered to correlate with tumor grade, and contribute to the malignant progression of bladder cancer. LOH in the P53 gene may serve as a clinical indicator for prognosis in superficial bladder cancer.

译文

背景:我们检查了膀胱癌中P53基因的杂合性(LOH)缺失,并研究了P53基因在乳头状瘤恶性进展中的作用。另外,检查了复发性膀胱癌的克隆性。
方法:分析了47例患者的67例膀胱癌中P53基因的LOH。从福尔马林固定,石蜡包埋的组织中提取DNA,在P53基因的3个多态性位点处通过聚合酶链反应(PCR)进行扩增,并通过非放射性同位素单链构象多态性(Non-RI SSCP)分析。
结果:在40个信息量样本中,在13个样本中检测到LOH,其中3个7级中有4个(57%),2个23级中有9个(39%),1个10级中没有10个(10%)。在1级和2级以及1级和3级的LOH之间观察到统计学意义。对5例恶性进展的分析表明,3例(60%)在原发性肿瘤中显示LOH,2例在复发性肿瘤中显示LOH。 ,与LOH在19例(16%)的3例中未显示出恶性进展的情况相反。 4例异时复发表现为LOH。在复发性肿瘤中2个,仅在初始肿瘤中1个,在两个肿​​瘤中1个。
结论:P53基因的改变被认为与肿瘤的分级有关,并有助于膀胱癌的恶性进展。 P53基因中的LOH可作为浅表性膀胱癌预后的临床指标。

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