Both extrinsic and intrinsic tissues contribute to tendon repair, but the origin and molecular functions of extrinsic tissues in tendon repair are not fully understood. Here we show that tendon sheath cells harbor stem/progenitor cell properties and contribute to tendon repair by activating Hedgehog signaling. We found that Osteocalcin (Bglap) can be used as an adult tendon-sheath-specific marker in mice. Lineage tracing experiments show that Bglap-expressing cells in adult sheath tissues possess clonogenic and multipotent properties comparable to those of stem/progenitor cells isolated from tendon fibers. Transplantation of sheath tissues improves tendon repair. Mechanistically, Hh signaling in sheath tissues is necessary and sufficient to promote the proliferation of Mkx-expressing cells in sheath tissues, and its action is mediated through TGFβ/Smad3 signaling. Furthermore, co-localization of GLI1+ and MKX+ cells is also found in human tendinopathy specimens. Our work reveals the molecular function of Hh signaling in extrinsic sheath tissues for tendon repair.

译文

:外在和内在组织都有助于肌腱修复,但外在组织在肌腱修复中的起源和分子功能尚不完全清楚。在这里,我们显示肌腱鞘细胞具有干/祖细胞特性,并通过激活刺猬信号来促进肌腱修复。我们发现骨钙蛋白(Bglap)可以用作小鼠中成年肌腱鞘的特异性标志物。谱系追踪实验表明,成年鞘组织中表达Bglap的细胞具有克隆形成和多能性的特性,与从肌腱纤维中分离的干/祖细胞的特性相当。鞘组织的移植改善了肌腱的修复。从机理上讲,鞘内组织中的Hh信号对于促进鞘组织中表达Mkx的细胞的增殖是必要和充分的,并且其作用是通过TGFβ/ Smad3信号介导的。此外,在人类肌腱病标本中还发现了GLI1和MKX细胞的共定位。我们的工作揭示了Hh信号在外在鞘组织中用于肌腱修复的分子功能。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录