We raised specific antisera against recombinant VAR2CSA domains produced in Escherichia coli and in insect cells. All were reactive in enzyme-linked immunosorbent assay, but only insect cell-derived constructs induced immunoglobulin G (IgG) that was reactive with native VAR2CSA on the surface of infected erythrocytes. Our data show that five of the six VAR2CSA Duffy-binding-like domains are surface exposed and that induction of surface-reactive VAR2CSA-specific IgG depends critically upon antigen conformation. These findings have implications for the development of vaccines against pregnancy-associated Plasmodium falciparum malaria.

译文

:我们针对在大肠杆菌和昆虫细胞中产生的重组VAR2CSA域产生了特异性抗血清。所有这些都在酶联免疫吸附测定中具有反应性,但是只有昆虫细胞衍生的构建体诱导了免疫球蛋白G(IgG),该蛋白与被感染红细胞表面的天然VAR2CSA具有反应性。我们的数据表明,六个VAR2CSA Duffy结合样结构域中的五个是表面暴露的,表面反应性VAR2CSA特异性IgG的诱导关键取决于抗原构象。这些发现对于开发与妊娠相关的恶性疟原虫疟疾的疫苗具有重要意义。

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