Proopiomelanocortin (POMC) is an important gene implicated in different functions, such as the stress response of the hypothalamus-pituitary-adrenal axis. The aim of the present study was to determine whether farming conditions, such as stocking density, can be considered a powerful stressor influencing in turn the growth rate in juvenile fish. Thus, POMC cDNA expression was investigated during adaptation to farming conditions in sole (Solea solea), as a model for studying the effects of rearing densities on stress response; different stocking densities (50, 100, and 250 animals/m(2)) were applied and, after 7 and 21 days, the fishes were examined for body weight and plasma cortisol levels as indicators of stress. In addition, proopiomelanocortin was cloned and sequenced from the brain of sole, allowing semi-quantitative RT-PCR to be performed to evaluate POMC mRNA expression in brain tissue. There was a significant increase in cortisol levels in fish reared at high stocking densities of 250/m(2) compared to fish reared at control densities of 100 and 50/m(2), in both experimental times, i.e., 7 and 21 days. The high stocking densities were also found to decrease the specific growth rate of fish. Moreover, it was demonstrated that the highest stocking density induced a significant decrease in sole POMC mRNA expression. It is concluded that POMC and cortisol are both involved in the stress response due to high rearing densities, during which cortisol may serve as a negative regulator of POMC.

译文

:Proopiomelanocortin(POMC)是一个重要的基因,涉及不同的功能,例如下丘脑-垂体-肾上腺轴的应激反应。本研究的目的是确定养殖条件,例如放养密度,是否可以被认为是反过来影响幼鱼生长速度的强大压力源。因此,在鞋底适应不同耕作条件的过程中,研究了POMC cDNA的表达(Solea solea),作为研究饲养密度对胁迫反应的影响的模型。应用不同的放养密度(50、100和250动物/平方米(2)),并在7天和21天后,检查鱼的体重和血浆皮质醇水平,作为压力指标。此外,从足底的大脑中克隆了proopiomelanocortin,并对其进行了测序,从而可以进行半定量RT-PCR来评估脑组织中POMC mRNA的表达。在两个实验时间内(即7天和21天),与以100和50 / m(2)的对照密度饲养的鱼相比,以250 / m(2)的高饲养密度饲养的鱼的皮质醇水平显着增加。 。还发现高放养密度降低了鱼类的比生长速度。而且,已经证明最高的放养密度引起单独的POMC mRNA表达的显着降低。结论是,由于高饲养密度,POMC和皮质醇都参与了应激反应,在此期间皮质醇可能是POMC的负调节剂。

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