Five isolates of coagulase-negative staphylococci were obtained from human urine, the gastrointestinal tract of squirrel monkeys, pig skin and from the environment. All key biochemical characteristics of the tested strains corresponded with the description of Staphylococcus xylosus species. However, partial 16S rRNA gene sequences obtained from analysed strains corresponded with those of Staphylococcus nepalensis reference strains, except for two strains which differed in one residue. Ribotyping with EcoRI and HindIII restriction enzymes, whole cell protein profile analysis performed by SDS-PAGE and SmaI macrorestriction analysis were used for more precise characterization and identification of the analysed strains. Obtained results showed that EcoRI and HindIII ribotyping and whole cell protein fingerprinting are suitable and reliable methods for the differentiation of S. nepalensis strains from the other novobiocin resistant staphylococci, whereas macrorestriction analysis was found to be a good tool for strain typing. The isolation of S. nepalensis is sporadic, and according to our best knowledge this study is the first report of the occurrence of this species in human clinical material as well as in other sources.

译文

:从人尿,松鼠的胃肠道,猪皮和环境中获得了五种凝固酶阴性葡萄球菌的分离株。被测菌株的所有关键生化特征均与葡萄球菌属物种的描述相对应。但是,从分析菌株获得的部分16S rRNA基因序列与尼泊尔葡萄球菌参考菌株相对应,除了两个菌株在一个残基上有所不同。使用EcoRI和HindIII限制性内切酶进行核糖体分型,通过SDS-PAGE和SmaI宏观限制性分析进行的全细胞蛋白质谱分析可用于更精确地表征和鉴定所分析的菌株。所得结果表明,EcoRI和HindIII核糖分型和全细胞蛋白质指纹图谱是区分尼泊尔链球菌菌株和其他对新霉素耐药的葡萄球菌的可靠方法,而宏观限制性分析被认为是很好的菌株分型工具。尼泊尔链球菌的分离是零星的,根据我们的最佳知识,这项研究是人类临床材料以及其他来源中该物种出现的第一份报告。

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