A study was conducted to determine whether following exposure of male mice to high temperatures, the ability of their spermatozoa to fertilise ova was reduced, especially during the period before the males became completely infertile. Male mice placed in a microclimate chamber at 36 degrees C for two periods, each of 12 h on successive days, were less able to fertilise control females in vivo when mated and, even in those females that became pregnant, litter size was reduced. However, these effects were associated with falls in testis weight and numbers of spermatozoa in the testis and epididymis. To determine whether the effect on fertility was a result of the decreased spermatozoa numbers, spermatozoa were collected from the epididymides of heated and control males. Equal numbers of motile spermatozoa from an unselected sample or those subjected to a swim-up procedure to separate those that were motile from the immotile ones in the sample were then mixed in vitro with oocytes from superovulated normal females. Similar numbers of spermatozoa from both control and heated males bound to the zona pellucida but smaller percentages of the oocytes were fertilised by spermatozoa from the heated males and fewer of these spermatozoa penetrated the ova. The effects were first seen 7 days after the heat exposure and became more obvious after 10 or 14 days.

译文

:进行了一项研究,以确定在雄性小鼠暴露于高温之后,其精子受精卵的能力是否降低,尤其是在雄性小鼠完全不育之前的时期。将雄性小鼠在36摄氏度的小气候室内放置两次,每次连续12h,连续交配时,它们在体内不能使对照雌性小鼠受精,即使在那些怀孕的雌性小鼠中,其产仔数也会减少。然而,这些影响与睾丸重量的下降以及睾丸和附睾中精子数量的减少有关。为了确定对生育力的影响是否是精子数量减少的结果,从加热和对照雄性的附睾中收集精子。然后,将来自未选择样品的等量活动精子或经过泳动程序分离出样品中不活动精子的活动精子与来自超排卵正常雌性的卵母细胞体外混合。结合在透明带上的对照雄性和受热雄性的精子数量相似,但是受热雄性的精子受精的卵母细胞受精的百分比较小,并且这些精子很少渗透到卵子中。在暴露于热的7天后首次见到这种效果,在10或14天后更加明显。

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