Marins, EF, Cabistany, L, Farias, CB, Dawes, J, and Del Vecchio, FB. Effects of personal protective equipment on metabolism and performance during an Occupational Physical Ability Test for Federal Highway Police officers. J Strength Cond Res 34(4): 1093-1102, 2020-Federal Highway Police (FHP) officers perform their duties while wearing personal protective equipment (PPE). Given the need for officers to wear PPE, it is important to understand the effects this additional load can have on occupational performance. The objective of this study was to evaluate the effects of load carriage on physiological, perceptual, and performance responses during an Occupational Physical Ability Test (OPAT). Thirteen male FHP officers completed an OPAT with PPE (WPPE, load = 12.0 kg) and without PPE (NPPE, load = 5.2 kg) in 2 separate sessions in a randomized and counterbalanced order. Metabolic responses (heart rate [HR] and its variability, and blood lactate concentration [Lac]), perceptual (rating of perceived exertion [RPE]), and OPAT performance were measured. Paired t and Wilcoxon tests were used to identify differences between conditions, and 2-way analysis of variance with Bonferroni post hoc was performed to analyze the results of Lac between the conditions. Occupational Physical Ability Test completion time was significantly greater in the WPPE condition compared with the NPPE condition (125.9 ± 13.8 vs. 118.3 ± 11 seconds, p < 0.01). Lac after the OPAT was higher in the NPPE condition compared with the WPPE (respectively 11.7 ± 2.7 vs. 9.6 ± 1.5 mmol·L, p < 0.05). The HRmax (NPPE = 180.5 ± 8.9 vs. WPPE = 178.3 ± 6.6 b·min, p = 0.44) and RPE (NPPE = 8.6 ± 0.8 vs. WPPE = 8.7 ± 1.0, p = 0.77) were not different between conditions. Personal protective equipment reduces performance in specific occupational circuit, changing Lac but without affecting HR and RPE. Strength and conditioning programs for FHP should aim to reduce the negative impact of additional load associate with wearing PPE.