The number of primordial germ cells (PGC) in albino tadpoles of Xenopus is significantly decreased as compared with that of the wild-type. Whether the decreased number of PGC is caused by the presumptive PGC (pPGC) themselves or the micro-environment surrounding those cells in the albino, or both was investigated in the present study. [3H]thymidine-labeled pPGC of wild-type and albino were implanted into unlabeled, host neurulae of wild-type or albino and wild-type, respectively. Labeled PGC in the genital ridges of experimental tadpoles were examined by autoradiography. There were no significant differences in the proportion of tadpoles with labeled PGC and in the average number of those PGC between the albino and wild-type tadpoles, into which wild-type pPGC had been implanted. The proportion in wild-type tadpoles with albino pPGC was much lower than that in wild-type tadpoles with wild-type pPGC. These results suggest that the pPGC of the albino and not the micro-environment are responsible for the decreased number of PGC.

译文

:与野生型相比,非洲爪蟾的白化t中原始生殖细胞(PGC)的数量明显减少。在本研究中,研究了PGC数量减少是由推测的PGC(pPGC)本身还是由白化病中那些细胞周围的微环境引起,还是两者都有。 [3 H]胸苷标记的野生型和白化病的pPGC分别植入未标记的,野生型或白化病和野生型的宿主神经元中。通过放射自显影检查实验t生殖脊中的标记PGC。植入了野生型pPGC的白化病和野生型between之间,带有标记的PGC的t比例和平均PGC数无显着差异。白化病pPGC的野生型ad的比例远低于白化病pPGC的野生型proportion的比例。这些结果表明,白化病的pPGC而不是微环境是造成PGC数量减少的原因。

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