A strictly aerobic, Gram-stain positive, slightly halophilic strain, designated SCSIO 04524(T), was isolated from a deep sea sediment sample collected from the northern South China Sea at a depth of 3415 m. The isolate slightly embedded into the medium after 72 h incubation at 30 °C. Growth was found to occur on media with 0-10 % NaCl but extremely weak growth occurred without supplying NaCl. The predominant menaquinone was determined to be MK-7. The major cellular fatty acid identified was iso-C15:0. The diagnostic polar lipids were determined to be diphosphatidylglycerol, phosphatidyl methylethanolamine, phosphatidylethanolamine and phosphatidylglycerol. The genomic DNA G+C content was determined to be 38 mol%. 16S rRNA gene sequences analysis showed that this strain had the highest similarities with Bacillus carboniphilus JCM 9731(T) (94.7 %) and Bacillus endophyticus 2DT(T) (94.3 %). Phylogenetic analysis revealed that strain SCSIO 04524(T) formed a distinct lineage with Bacillus chungangensis CAU 348(T) and B. carboniphilus JCM 9731(T). Physiological characteristics including utilization of sole nitrogen and carbon sources, and chemotaxonomic properties of cellular fatty acids and polar lipids could readily distinguish strain SCSIO 04524(T) from its most closely related species. Based on this polyphasic taxonomic data, a new species, Bacillus oceani sp. nov., is proposed, with the type strain SCSIO 04524(T) (=DSM 26213(T) = KCTC 33077(T)).

译文

:从南海北部3415 m深度的深海沉积物样本中分离出一种严格需氧的革兰氏染色阳性,略嗜盐的菌株,命名为SCSIO 04524(T)。在30°C下孵育72小时后,分离株稍微嵌入培养基中。发现在含0-10%NaCl的培养基上发生生长,但不供应NaCl则发生极弱的生长。确定主要的甲萘醌为MK-7。鉴定出的主要细胞脂肪酸是iso-C15:0。诊断极性脂质被确定为二磷脂酰甘油,磷脂酰甲基乙醇胺,磷脂酰乙醇胺和磷脂酰甘油。基因组DNA G C含量测定为38摩尔%。 16S rRNA基因序列分析表明,该菌株与嗜碳芽孢杆菌JCM 9731(T)(94.7%)和内生芽孢杆菌2DT(T)(94.3%)具有最高的相似性。系统发育分析表明,菌株SCSIO 04524(T)与春芽孢杆菌CAU 348(T)和炭疽芽孢杆菌JCM 9731(T)形成了独特的谱系。包括利用唯一的氮和碳源的生理特性,以及细胞脂肪酸和极性脂质的化学分类学特性,很容易将SCSIO 04524(T)菌株与其最密切相关的物种区分开。基于该多相分类学数据,一个新物种海洋芽孢杆菌(Bacillus oceani sp。)。提出了nov。,类型为SCSIO 04524(T)(= DSM 26213(T)= KCTC 33077(T))。

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