Extended-spectrum beta-lactamase (ESBL) and AmpC-producing Enterobacteriaceae are an increasing problem in human medicine and an emerging problem in the veterinary field. Our study, therefore, focused on assessing the prevalence of beta-lactamases isolated from swine. Sixty-six Salmonella enterica serovar Typhimurium (S. Typhimurium), 33 Salmonella enterica serovar Enteritidis (S. Enteritidis), 26 Klebsiella pneumonia (K. pneumoniae) and 130 Escherichia coli (E. coli) pig isolates collected from 1999-2006 were screened for beta-lactam resistance by the disk diffusion test (DDT) and micro-broth dilution. Among the isolates, five E. coli and five K. pneumoniae exhibited reduced susceptibility to the cephalosporins tested. PCR, plasmid profiling and Southern blot hybridization showed the presence of multiple beta-lactamases in these isolates of animal origin. Hybridization patterns of the DHA-1 specific probe indicated that dissemination of DHA-1 related beta-lactamases could be attributed to plasmids of one common size among the enteric microbes of animal origin. To the best of our knowledge, this study reports the first identification of SHV-28 and DHA-1 from microbes of animal origin.

译文

:广谱β-内酰胺酶(ESBL)和产生AmpC的肠杆菌科是人类医学中一个日益严重的问题,也是兽医领域中一个新出现的问题。因此,我们的研究集中在评估从猪中分离出的β-内酰胺酶的患病率。筛选了1999年至2006年收集的66株肠炎沙门氏菌鼠伤寒沙门氏菌(S. Typhimurium),33株肠炎沙门氏菌肠炎沙门氏菌(S. Enteritidis),26株肺炎克雷伯菌(K. pneumoniae)和130株猪大肠杆菌。通过圆盘扩散测试(DDT)和微肉汤稀释获得的β-内酰胺抗性。在分离物中,有五种大肠杆菌和五种肺炎克雷伯菌显示出对所测试的头孢菌素的敏感性降低。 PCR,质粒分析和Southern印迹杂交显示在这些动物来源的分离物中存在多种β-内酰胺酶。 DHA-1特异性探针的杂交模式表明,与DHA-1相关的β-内酰胺酶的传播可归因于动物来源的肠道微生物中一种常见大小的质粒。据我们所知,本研究报告首次从动物来源的微生物中鉴定出SHV-28和DHA-1。

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