High productivity processes are critical for commercial production of cellulosic ethanol. One high productivity process-continuous hydrolysis and fermentation-has been applied in corn ethanol industry. However, little research related to this process has been conducted on cellulosic ethanol production. Here, we report and compare the kinetics of both batch SHF (separate hydrolysis and co-fermentation) and SSCF (simultaneous saccharification and co-fermentation) of AFEX™ (Ammonia Fiber Expansion) pretreated corn stover (AFEX™-CS). Subsequently, we designed a SSCF process to evaluate continuous hydrolysis and fermentation performance on AFEX™-CS in a series of continuous stirred tank reactors (CSTRs). Based on similar sugar to ethanol conversions (around 80% glucose-to-ethanol conversion and 47% xylose-to-ethanol conversion), the overall process ethanol productivity for continuous SSCF was 2.3- and 1.8-fold higher than batch SHF and SSCF, respectively. Slow xylose fermentation and high concentrations of xylose oligomers were the major factors limiting further enhancement of productivity.

译文

:高生产率工艺对于纤维素乙醇的商业化生产至关重要。一种高生产率的过程-连续水解和发酵-已应用于玉米乙醇工业。但是,有关纤维素乙醇生产的研究很少与此过程有关。在这里,我们报告并比较了AFEX™(氨纤维膨胀)预处理玉米秸秆(AFEX™-CS)的批次SHF(单独的水解和共同发酵)和SSCF(同时糖化和共同发酵)的动力学。随后,我们设计了一种SSCF工艺,以评估一系列连续搅拌釜反应器(CSTR)在AFEX™-CS上的连续水解和发酵性能。基于相似的糖到乙醇转化率(大约80%的葡萄糖到乙醇转化率和47%的木糖到乙醇转化率),连续SSCF的总工艺乙醇生产率比批次SHF和SSCF高2.3到1.8倍,分别。木糖发酵缓慢和木糖低聚物浓度高是限制生产率进一步提高的主要因素。

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