The distribution of endothelin-1 (ET-1) and endothelin-3 (ET-3) was studied by indirect immunostaining of decalcified guinea pig and rat cochleae. No species differences were observed. Perikarya and processes of spiral ganglion cells were highly reactive for both ET-1 and ET-3. The epithelial lining of the cochlear duct stained for ET-1 and ET-3, but reactivity for ET-1 was higher in the lining cells of the inner sulcus, Claudius', and Hensen's cells, while the tympanic covering layer of the basilar membrane stained stronger for ET-3 compared to ET-1. In the stria vascularis, all cell types stained for ET-3, while marginal cells were more reactive for ET-1. Spiral ligament fibroblasts were reactive for ET-1, but not for ET-3. Connective tissue cells of the spiral limbus stained for both endothelins. The region of synapses on outer hair cells reacted for ET-1 and ET-3 but sensory cells remained unstained. Endothelins are discussed to act as modulatory peptides, possibly interfering with nitric oxide, prostaglandins, and atrial natriuretic peptide in the lateral cochlear wall (lateral cochlear wall, i.e. stria vascularis and spiral ligament). The occurrence of endothelins in cochlear neurons suggest their potential role as neurotransmitters.

译文

通过对脱钙的豚鼠和大鼠耳蜗进行间接免疫染色,研究了内皮素-1(ET-1)和内皮素3(ET-3)的分布。没有观察到物种差异。蠕周和螺旋神经节细胞的过程对ET-1和ET-3都具有高反应性。耳蜗上皮衬有ET-1和ET-3染色,但内沟,Claudius和Hensen细胞的衬里细胞对ET-1的反应性较高,而基底膜的鼓膜覆盖层与ET-1相比,ET-3的染色更强。在血管纹中,所有细胞类型均对ET-3染色,而边缘细胞对ET-1更具反应性。螺旋韧带成纤维细胞对ET-1有反应,但对ET-3没有反应。螺旋角膜缘的结缔组织细胞均被两种内皮素染色。外毛细胞上的突触区域对ET-1和ET-3反应,但感觉细胞仍未染色。讨论了内皮素作为调节肽,可能会干扰侧耳壁(侧耳壁,即血管纹和螺旋韧带)中的一氧化氮,前列腺素和心钠素。耳蜗神经元中内皮素的出现表明它们可能作为神经递质。

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