The immune-releasing effects of L-glutamine (Gln) supplementation in duck plague virus (DPV)-infected ducklings were evaluated in 120 seven-day-old ducklings that were divided into 8 groups. The ducklings in control and DPV, 0.5Gln and DPV + 0.5Gln, 1.0Gln and DPV + 1.0Gln, and 2.0Gln and DPV + 2.0Gln received 0, 0.5, 1.0, and 2.0 g of Gln/kg feed/d by gastric perfusion, respectively. Then, the ducklings in control to 2.0Gln were injected with 0.2 mL of phosphate-buffered saline, while those in DPV to DPV + 2.0Gln were injected with DPV at 0.2 mL of 2000 TCID50 (50% tissue culture infection dose) 30 minutes after gavage with Gln, sampled at 12 hours and days 1, 2, 4, and 6. Glutamine supplementation under physiological conditions enhanced immune function and toll-like receptor 4 (TLR4) expressions in a dose-dependent manner. An increase in Gln supplementation under DPV-infected conditions enhanced growth performance, decreased immunoglobulin (Ig) release in plasma and secretory IgA in the duodenum, ameliorated plasma cytokine levels, and suppressed overexpressions of the TLR4 pathway in the duodenum. The positive effects of Gln on the humoral immunity- and intestinal inflammation-related damage should be considered a mechanism by which immunonutrition can assist in the recovery from DPV infection.

译文

:在120只7日龄小鸭中,分为8组,评估了补充L-谷氨酰胺(Gln)对鸭瘟病毒(DPV)感染的小鸭的免疫释放作用。对照和DPV,0.5Gln和DPV 0.5Gln,1.0Gln和DPV 1.0Gln以及2.0Gln和DPV 2.0Gln的小鸭通过胃灌注分别获得0、0.5、1.0和2.0 g Gln / kg饲料。然后,在管饲后30分钟,向对照组的2.0Gln的小鸭注射0.2 mL磷酸盐缓冲液,向DPV至DPV 2.0Gln的小鸭注射0.2 mL的2000 TCID50(50%组织培养物感染剂量)的DPV。在生理条件下补充谷氨酰胺在12小时和第1、2、4和6天取样,以剂量依赖的方式增强了免疫功能和toll样受体4(TLR4)的表达。在DPV感染的条件下增加Gln的添加可以增强生长性能,降低十二指肠血浆中的免疫球蛋白(Ig)释放和分泌型IgA,改善血浆细胞因子水平,并抑制十二指肠TLR4途径的过表达。 Gln对与体液免疫和肠道炎症相关的损害的积极作用应被认为是免疫营养可以帮助从DPV感染中恢复的机制。

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