Pseudomonas putida U does not degrade D-glucose through the glycolytic pathway but requires (i) its oxidation to D-gluconic acid by a peripherally located constitutive glucose dehydrogenase (insensitive to osmotic shock), (ii) accumulation of D-gluconic acid in the extracellular medium, and (iii) the induction of a specific energy-dependent transport system responsible for the uptake of D-gluconic acid. This uptake system showed maximal rates of transport at 30 degrees C in 50 mM potassium phosphate buffer, pH 7.0. Under these conditions the K(m) calculated for D-gluconic acid was 6.7 microM. Furthermore, a different transport system, specific for the uptake of glucose, was also identified. It is active and shows maximal uptake rates at 35 degrees C in 50 mM potassium phosphate buffer, pH 6.0, with a K(m) value of 8.3 microM.

译文

恶臭假单胞菌U不会通过糖酵解途径降解D-葡萄糖,但需要(i)通过位于外围的组成型葡萄糖脱氢酶(对渗透压休克不敏感)将其氧化为D-葡萄糖酸,(ii)D-葡萄糖酸在D.葡萄糖中的积累(iii)诱导负责摄取D-葡萄糖酸的特定能量依赖性转运系统。该摄取系统显示了在30 mC,pH 7.0的50 mM磷酸钾缓冲液中的最大运输速率。在这些条件下,D-葡萄糖酸的K(m)计算值为6.7 microM。此外,还发现了一个专门针对葡萄糖吸收的不同转运系统。它是有活性的,在35 m的50 mM磷酸钾缓冲液(pH 6.0)中显示最大吸收速率,K(m)值为8.3 microM。

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