Development of methodologies for gene transfer into the central nervous system (CNS) is important for fundamental research as well as clinical studies for gene therapy. Cationic liposomes (CL) are attractive vectors because of their safety and ease of use. However, to date only low rates of success have been reported. We succeeded in obtaining high transfection efficiencies into the newborn mouse brain in vivo by CL and a cytoplasmic gene expression system based on T7 RNA polymerase and T7 RNA polymerase- and the luciferase-gene with the T7 promoter sequence. This system showed an efficiency rate 2 orders of magnitude higher than the standard system, which used CL and luciferase genes with a Rous sarcoma virus promoter, pRSVL. In addition, in vitro experiments using LLCMK2 cells showed that cytoplasmic gene expression occurred rapidly (within 6 h) after transfection. In contrast, pRSVL required 24-48 h for induction of luciferase expression. Our results suggest that the cytoplasmic gene expression system is useful for gene delivery into the CNS.

译文

基因转移到中枢神经系统(CNS)的方法学的发展对于基础研究以及基因治疗的临床研究都很重要。由于阳离子脂质体(CL)的安全性和易用性,它们是有吸引力的载体。然而,迄今为止,仅报道了低成功率。我们成功地通过CL和基于T7 RNA聚合酶和T7 RNA聚合酶以及荧光素酶基因(具有T7启动子序列)的细胞质基因表达系统,成功地在体内获得了新生小鼠大脑的高转染效率。该系统的效率比标准系统高2个数量级,后者使用带有Rous肉瘤病毒启动子pRSVL的CL和荧光素酶基因。此外,使用LLCMK2细胞的体外实验显示,转染后(6小时内)细胞质基因表达迅速发生。相比之下,pRSVL需要24-48小时才能诱导萤光素酶表达。我们的结果表明,胞质基因表达系统可用于将基因传递到中枢神经系统。

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