Cyanobacteria inhabit nearly every ecosystem on earth, play a vital role in nutrient cycling, and are useful as model organisms for fundamental research in photosynthesis and carbon and nitrogen fixation. In addition, they are important for several established biotechnologies for producing food additives, nutritional and pharmaceutical compounds, and pigments, as well as emerging biotechnologies for biofuels and other products. Encapsulation of living cyanobacteria into a porous silica gel matrix is a recent approach that may dramatically improve the efficiency of certain production processes by retaining the biomass within the reactor and modifying cellular metabolism in helpful ways. Although encapsulation has been explored empirically in the last two decades for a variety of cell types, many challenges remain to achieving optimal encapsulation of cyanobacteria in silica gel. Recent evidence with Synechocystis sp. PCC 6803, for example, suggests that several unknown or uncharacterized proteins are dramatically upregulated as a result of encapsulation. Also, additives commonly used to ease stresses of encapsulating living cells, such as glycerol, have detrimental impacts on photosynthesis in cyanobacteria. This mini-review is intended to address the current status of research on silica sol-gel encapsulation of cyanobacteria and research areas that may further the development of this approach for biotechnology applications.

译文

:蓝藻几乎生活在地球上的每个生态系统中,在养分循环中起着至关重要的作用,并且可以作为模型生物用于光合作用和碳氮固定的基础研究。此外,它们对于生产食品添加剂,营养和药物化合物以及色素的几种已确立的生物技术以及用于生物燃料和其他产品的新兴生物技术也很重要。将活性蓝细菌封装到多孔硅胶基质中是一种最近的方法,该方法可以通过将生物质保留在反应器中并以有用的方式修饰细胞代谢来显着提高某些生产过程的效率。尽管在过去的二十年中对各种细胞类型进行了封装研究,但是在硅胶中实现蓝细菌的最佳封装仍然存在许多挑战。最近的证据与集胞藻。例如,PCC 6803建议,由于封装,几种未知或未表征的蛋白质会显着上调。而且,通常用于减轻封装活细胞的压力的添加剂,例如甘油,对蓝细菌的光合作用具有有害影响。这份小型综述旨在解决蓝藻细菌二氧化硅溶胶-凝胶封装的研究现状以及可能会进一步发展该方法用于生物技术应用的研究领域。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录