The swelling behavior of the structural material of cell-enclosing capsules is a key factor for the successful transplantation of these capsules in the treatment of diseases. The present study aimed to develop cell-enclosing capsules displaying minimal swelling under physiological conditions. We investigated the use of an alginate-tyramine conjugate synthesized by a carbodiimide/active-ester coupling reaction. The conjugate gel crosslinked by calcium ions and peroxidase-catalyzed oxidative coupling of phenols swelled less in saline than in unmodified alginate. The degree of swelling could be controlled by conjugate preparation conditions. The conjugate gel showed no obvious cytotoxicity for cells, including the process of oxidative coupling generation. Further, encapsulated cells could be cultured for up to 2 months and achieve approximately 5.2-fold greater mitochondrial activity after 51 days of encapsulation than after 1 day. These results show that this alginate-tyramine conjugate is a promising material for use in cell-enclosing capsules for cell therapy.

译文

:封闭细胞的胶囊的结构材料的溶胀行为是这些胶囊在疾病治疗中成功移植的关键因素。本研究旨在开发在生理条件下显示最小溶胀的封闭细胞的胶囊。我们研究了通过碳二亚胺/活性酯偶联反应合成的藻酸盐-酪胺共轭物的使用。通过钙离子交联的共轭凝胶和过氧化物酶催化的酚氧化偶联在盐水中的溶胀少于未改性藻酸盐中的溶胀。溶胀度可以通过结合物的制备条件来控制。共轭凝胶对细胞没有明显的细胞毒性,包括氧化偶合过程。此外,被包封的细胞可以培养长达2个月,并且在包封51天后的线粒体活性比在1天后提高约5.2倍。这些结果表明,该藻酸盐-酪胺结合物是用于细胞治疗细胞的封闭胶囊中的有前途的材料。

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