An enzyme-linked immunosorbent assay (ELISA) was developed which permits the assay of specific secretory immunoglobulin A (sIgA) antibodies against hepatitis B surface antigen (HBsAg) in saliva. The assay is based on the binding of sIgA antibodies present in saliva to microtitre plates coated with excess of F(ab')2 anti-secretory component antibodies, followed by the addition of specific antigen, HBsAg and finally peroxidase-labelled anti-HBsAg. The assay is fast, simple, reproducible and antigen specific as shown by total absence of inhibition of specific antigen by unrelated antigens but significant inhibition of labelled anti-HBsAg by unlabelled anti-HBsAg. The values obtained for hospital personnel exposed to hepatitis infections (0.068 +/- 0.083 U/ml) and for post-icteric hepatitis B patients (0.062 +/- 0.033 U/ml) were significantly higher than values in control subjects (0.013 +/- 0.006 U/ml).

译文

:开发了一种酶联免疫吸附测定(ELISA),可以测定唾液中针对乙型肝炎表面抗原(HBsAg)的特异性分泌型免疫球蛋白A(sIgA)抗体。该测定基于唾液中存在的sIgA抗体与包被有过量F(ab')2抗分泌成分抗体的微量滴定板的结合,然后添加特异性抗原HBsAg,最后添加过氧化物酶标记的抗HBsAg。该测定是快速,简单,可重现的和抗原特异性的,如完全不存在不相关抗原对特异性抗原的抑制,但未标记抗-HBsAg对标记抗-HBsAg的显着抑制所示。暴露于肝炎感染的医院工作人员获得的值(0.068 /-0.083 U / ml)和黄疸型乙型肝炎患者获得的值(0.062 /-0.033 U / ml)显着高于对照受试者的值(0.013 /-0.006 U / ml)。

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