In the present study, we describe the generation of a series of anti-interferon-alpha8 (IFN-alpha8)-specific monoclonal antibodies (mAbs), their characterization, and the establishment of a sandwich enzyme-linked immunosorbent assay (ELISA) system for human IFN-alpha8. The sandwich ELISA system is highly sensitive to human natural IFN-alpha8 (nIFN-alpha8), with a minimum detection limit of 50 pg/mL, which did not cross-react with the other IFN preparations and several cytokines tested. Using this ELISA system, pharmacokinetic properties of an IFN-alpha preparation administered in mice were examined. We found that IFN-alpha8 has higher vascular permeability and stability than IFN-alpha2 in the circulation. These results suggest that this ELISA would be very useful for determination of IFN-alpha8 protein concentrations in various experimental samples and also of pharmacokinetic properties of IFN-alpha preparations in human.

译文

:在本研究中,我们描述了一系列抗干扰素-α8(IFN-α8)特异性单克隆抗体(mAbs)的产生,其表征以及夹心酶联免疫吸附测定(ELISA)系统的建立对于人IFN-α8。夹心ELISA系统对人天然IFN-α8(nIFN-alpha8)高度敏感,最低检测限为50 pg / mL,与其他IFN制剂和几种测试的细胞因子没有交叉反应。使用该ELISA系统,检查了在小鼠中施用的IFN-α制剂的药代动力学特性。我们发现,IFN-alpha8在循环中比IFN-alpha2具有更高的血管通透性和稳定性。这些结果表明,该ELISA对于测定各种实验样品中的IFN-α8蛋白浓度以及人中的IFN-α制剂的药代动力学特性将非常有用。

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