OBJECTIVE:To determine the degree of correlation between Farr and ELISA methods of detecting anti-dsDNA antibodies in patients with systemic lupus erythematosus (SLE), and their association with measures of disease activity. METHODS:Anti-dsDNA antibodies were assayed using the Farr and ELISA methods in patients followed between January 1, 2000, and December 31, 2002. Statistical correlations between Farr and ELISA were determined. Relationships between the 2 assays and measures of disease activity [SLE Disease Activity Index 2000 (SLEDAI-2K-DNA), renal, central nervous system (CNS), and vasculitis] were determined for the same clinic visit. RESULTS:550 patients with 2940 clinic visits met the inclusion criteria. Correlation between Farr and ELISA levels was 0.46 using the first visit for each patient. When the Farr was abnormal, the ELISA was equally likely to be normal or abnormal. Abnormal Farr results were associated with higher SLEDAI-2K scores than normal Farr results (6.2 vs 4.3, respectively; p < 0.0001). There was less of a distinction with ELISA results (5.9 vs 4.8; p = 0.04). Farr levels were significantly associated with the presence of renal disease and vasculitis, while ELISA levels were not. Neither Farr nor ELISA results correlated with the presence of active CNS involvement. CONCLUSION:Farr and ELISA techniques for the detection of anti-dsDNA antibodies in patients with SLE are poorly correlated. The Farr is superior to the ELISA in correlating with measures of global disease activity, as well as renal and vasculitis involvement. The Farr technique should continue to be used in clinical practice. The ELISA adds no additional information.

译文

目的:探讨系统性红斑狼疮(SLE)患者中Farr法和ELISA法检测抗dsDNA抗体之间的相关程度,以及它们与疾病活动性的相关性。
方法:在2000年1月1日至2002年12月31日之间,采用Farr和ELISA方法对患者的抗dsDNA抗体进行检测。在同一门诊就诊时,确定了两种测定与疾病活动度之间的关系[SLE疾病活动指数2000(SLEDAI-2K-DNA),肾,中枢神经系统(CNS)和血管炎]。
结果:550名患者进行了2940次门诊就诊,符合入选标准。首次就诊时,Farr和ELISA水平之间的相关性为0.46。当Farr异常时,ELISA同样可能是正常的或异常的。与正常的Farr结果相比,异常的Farr结果与更高的SLEDAI-2K得分相关(分别为6.2和4.3; p <0.0001)。 ELISA结果几乎没有区别(5.9 vs 4.8; p = 0.04)。 Farr水平与肾脏疾病和血管炎的存在显着相关,而ELISA水平则不相关。 Farr或ELISA结果均与主动中枢神经系统受累无关。
结论:远距离和ELISA技术检测SLE患者抗dsDNA抗体的相关性较弱。 Farr在整体疾病活动以及肾脏和血管炎受累方面具有优于ELISA的能力。 Farr技术应继续在临床实践中使用。 ELISA没有添加其他信息。

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