Electrospun scaffolds incorporated with both calcium phosphates (Ca-P) and bone morphogenetic protein-2 (BMP-2) have been used for bone tissue regeneration. However, in most cases BMP-2 and Ca-P were simply mixed and loaded in a monolithic structure, risking low BMP-2 loading level, reduced BMP-2 biological activity, uncontrolled BMP-2 release and inhomogeneous Ca-P distribution. In this investigation, novel bicomponent scaffolds having evenly distributed rhBMP-2-containing fibers and Ca-P nanoparticle-containing fibers were made using an established dual-source dual-power electrospinning technique with the assistance of emulsion electrospinning and blend electrospinning. The release behavior of rhBMP-2 and Ca2+ ions could be separately tuned and the released rhBMP-2 retained a 68% level for biological activity. MC3T3-E1 cells showed high viability and normal morphology on scaffolds. Compared to monocomponent scaffolds, enhanced cell proliferation, alkaline phosphatase activity, cell mineralization, and gene expression of osteogenic markers were achieved for bicomponent scaffolds due to the synergistic effect of rhBMP-2 and Ca-P nanoparticles. Bicomponent scaffolds with a double mass elicited further enhanced cell adhesion, spreading, proliferation, and osteogenic differentiation. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 2199-2209, 2017.

译文

:结合磷酸钙(Ca-P)和骨形态发生蛋白2(BMP-2)的电纺支架已用于骨骼组织再生。但是,在大多数情况下,BMP-2和Ca-P只是简单地混合并以整体结构装载,存在BMP-2装载水平低,BMP-2生物活性降低,BMP-2释放不受控制以及Ca-P分布不均匀的风险。在这项研究中,利用乳液电纺和共混电纺的成熟双源双功率电纺技术,制备了具有均匀分布的含rhBMP-2纤维和含Ca-P纳米粒子的纤维的新型双组分支架。可以分别调节rhBMP-2和Ca2离子的释放行为,释放的rhBMP-2保留68%的生物活性。 MC3T3-E1细胞在支架上显示出高活力和正常形态。与单组分支架相比,由于rhBMP-2和Ca-P纳米颗粒的协同作用,双组分支架实现了增强的细胞增殖,碱性磷酸酶活性,细胞矿化和成骨标记基因表达。具有双重质量的双组分支架引发了进一步增强的细胞粘附,扩散,增殖和成骨分化。分级为4 +©2017 Wiley Periodicals,Inc.J Biomed Mater Res Part A:105A:2199-2209,2017。

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