Chondrogenesis occurs in vivo in a hypoxic environment, in which the hypoxia inducible factor 1, HIF-1, plays a regulatory role, possibly mediated through the transcription factor DEC1. We have analyzed the effect of hypoxia (1% oxygen) alone and in combination with insulin on the chondrogenic differentiation of the mouse embryonic stem cell line ATDC5. Hypoxic treatment alone induced early chondrogenesis as evidenced by enhanced expression of aggrecan and collagen II, whereas hypoxic incubation of insulin-treated cells delayed and suppressed insulin-mediated early chondrogenesis and almost completely blocked hypertrophic differentiation. Paradoxically, the transcriptional activation of DEC1 was invariably enhanced by the hypoxic exposure.

译文

:软骨形成发生在体内低氧环境中,在该环境中,缺氧诱导因子1(HIF-1)发挥调节作用,可能是通过转录因子DEC1介导的。我们已经分析了单独和与胰岛素联合使用低氧(1%氧气)对小鼠胚胎干细胞系ATDC5软骨分化的影响。单用低氧治疗可诱导早期软骨形成,聚集蛋白聚糖和胶原II的表达增强证明了这一点,而胰岛素处理细胞的低氧培养可延迟和抑制胰岛素介导的早期软骨形成,并且几乎完全阻断了肥大细胞的分化。矛盾的是,低氧暴露总是增强了DEC1的转录激活。

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