Isolated adrenal cells from Vitamin E-deficient and control rats were prepared by a trypsin digestion method. Cyclic adenosine 3',5'-monophosphate (cyclic AMP) formation was studied in response to adrenocorticotropin (ACTH) in the presence and absence of ascorbate by measuring the conversion of prelabeled adenosine 5'-triphosphate [14C]ATP to cyclic [14C]AMP. Ascorbate (0.5 mM) inhibited ACTH-induced cyclic [14C]AMP formation in adrenal cells isolated from Vitamin E-deficient rats but had no effect in the control cells. The inhibitory effect of ascorbate on ACTH-induced cyclic AMP formation in Vitamin E-deficient rats decreased as the concentration of ACTH increased. In Vitamin E-deficient rats ascorbate inhibited ACTH-induced cyclic [14C]AMP formation after 30 min of incubation. There was no further significant accumulation of cyclic [14C]AMP at 60 min or 120 min although in the absence of ascorbate cyclic [14C]AMP continued to be formed. The in vitro addition of alpha-tocopherol reduced the inhibition of ACTH-induced cyclic [14C]AMP formation by ascorbate in Vitamin E-deficient rats. These studies suggest that alpha-tocopherol and ascorbate may affect ACTH-induced cyclic AMP formation through interaction with the membrane-bound enzyme adenylate cyclase.

译文

:通过胰蛋白酶消化方法,从维生素E缺乏和对照大鼠中分离出肾上腺细胞。通过测量预先标记的5'-三磷酸腺苷[14C] ATP向环状[14C]的转化,研究了存在和不存在抗坏血酸时对肾上腺皮质激素(ACTH)的响应,研究了环状腺苷3',5'-单磷酸(环状AMP)的形成AMP。抗坏血酸(0.5 mM)抑制ACTH诱导的从维生素E缺乏症大鼠分离的肾上腺细胞中环[14C] AMP的形成,但对对照细胞没有作用。随着ACTH浓度的增加,抗坏血酸对ACTH诱导的维生素E缺乏症大鼠环AMP形成的抑制作用降低。在维生素E缺乏的大鼠中,孵育30分钟后,抗坏血酸会抑制ACTH诱导的环状[14C] AMP的形成。尽管在不存在抗坏血酸盐的情况下仍继续形成环状[14C] AMP,但在60分钟或120分钟时没有进一步显着的环状[14C] AMP蓄积。在维生素E缺乏的大鼠中,体外添加α-生育酚可降低抗坏血酸对ACTH诱导的环[14C] AMP形成的抑制作用。这些研究表明,α-生育酚和抗坏血酸可能通过与膜结合酶腺苷酸环化酶相互作用而影响ACTH诱导的环AMP的形成。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录