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Low intensity ultrasound signals, similar to that employed in clinical therapy, are found to mediate differential gene transfer and expression of the Green Fluorescence Protein (GFP) reporter in two human prostate cancer cell lines, LnCap and PC-3. Cell suspensions in the presence or in the absence of GFP (44.5nM) were treated at 37 degrees C under a standing wave condition. Cells were exposed to either continuous wave, 932.7kHz ultrasound, or to several independent bursts, each burst comprising a 20% duty cycle (932.7kHz) sine wave. The burst "repetition" frequency was varied from 10Hz to 10kHz in several different experiments and each treatment received a net identical ultrasound energy exposure. Transient GFP expression levels in viable cells were monitored by flow cytometry. The findings revealed a strong ultrasound tone-burst frequency dependence on the transfection efficiencies. Interestingly, the ultrasound signal parameters which are routinely employed in clinical therapy did not yield any statistically significant enhancement in transfection efficiency relative to their sham counterparts.

译文

发现低强度超声信号类似于临床治疗中使用的信号,可在两种人前列腺癌细胞系LnCap和PC-3中介导差异基因转移和绿色荧光蛋白(GFP)报告基因的表达。将存在或不存在GFP(44.5nM)的细胞悬浮液在37°C的驻波条件下进行处理。细胞暴露于连续波,932.7kHz超声或几个独立的脉冲串中,每个脉冲串包含20%占空比(932.7kHz)的正弦波。在几个不同的实验中,猝发的“重复”频率在10Hz到10kHz之间变化,并且每种处理都受到净相同的超声能量暴露。通过流式细胞术监测存活细胞中的瞬时GFP表达水平。这些发现揭示了超声音-猝发频率对转染效率的强烈依赖性。有趣的是,相对于假手术,常规用于临床治疗的超声信号参数在转染效率上没有产生任何统计学上的显着提高。

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