AIMS:Allergic eye disease affects up to 20% of the population with varying severity. The conjunctival epithelium plays a key role in allergic eye disease. The purpose of this study was to determine whether the conjunctival epithelium is abnormal in allergic eye disease. METHODS:Conjunctival biopsy samples were taken from patients with seasonal allergic conjunctivitis (SAC) 'in' and 'out of season' and nonatopic control subjects. Specimens were fixed in glycol methacrylate, 2 microm serial sections cut and Image-J used to assess the sites and areas of immuno-staining. RESULTS:E-cadherin, CD44, keratins K5/6, K8, K13, K14, K18 and pan-keratin immuno-staining were all significantly lower in patients 'out of season' compared with normal controls. No structural differences in the epithelium were observed between the two groups. The epithelium of patients 'in season' was thicker and immuno-staining of the above markers similar to controls. CONCLUSIONS:The expression of a wide spectrum of epithelial cell adhesion proteins and cytoskeletal elements is downregulated in the conjunctiva of SAC patients 'out of season' compared with normal controls. We suggest that this could have an important impact on the ability of the epithelium to protect itself against allergen penetration, potentially influencing the development and course of allergic eye disease and offering a novel area for therapeutic control.

译文

目的:过敏性眼病可影响多达20%的人口,其严重程度各不相同。结膜上皮在过敏性眼病中起关键作用。这项研究的目的是确定在过敏性眼病中结膜上皮是否异常。
方法:结膜活检样本取自“季节内”和“季节外”的季节性过敏性结膜炎(SAC)患者以及非特应性对照者。将样品固定在甲基丙烯酸乙二醇酯中,切成2微米的连续切片,用Image-J评估免疫染色的部位和面积。
结果:与正常对照组相比,“非季节性”患者的E-钙粘蛋白,CD44,角蛋白K5 / 6,K8,K13,K14,K18和泛角蛋白免疫染色均显着降低。两组之间未观察到上皮的结构差异。 “季节”患者的上皮较厚,上述标记物的免疫染色与对照相似。
结论:与正常对照组相比,SAC患者结膜中的各种上皮细胞粘附蛋白和细胞骨架成分的表达被“下调”。我们建议这可能对上皮保护自身免受过敏原渗透的能力产生重要影响,从而可能影响过敏性眼病的发展和进程,并为治疗控制提供一个新领域。

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