A nested PCR assay was employed to detect the presence of phytoplasmas in 127 blueberry plants exhibiting typical or a portion of blueberry stunt (BBS) syndrome collected in 2010 and 2011, from 11 commercial farms predominantly located in two counties in New Jersey, USA. Ninety plants exhibiting typical stunt syndrome tested positive for phytoplasma infection. Restriction fragment length polymorphism (RFLP) analysis indicated that two distinct phytoplasmas were associated with BBS-diseased plants. About 95% of phytoplasmas detected were very closely related to BBS phytoplasma strains BBS3-AR (subgroup 16SrI-E) and BBS1-MI (unidentified) identified previously, and 4.4% of phytoplasmas detected belonged to the pigeon pea witches'-broom phytoplasma group (16SrIX). Sequence and phylogenetic analysis of cloned 16S rDNA further indicated the subgroup 16SrI-E related phytoplasmas represented a variant of 16SrI-E reference strain BBS3-AR, while the 16SrIX related phytoplasmas were closely related to juniper witches'-broom (JunWB) phytoplasma (16SrIX-E), representing a 16SrIX-E variant. Ribosomal protein (rp) and secY gene-based phylogenies revealed that BBS3-AR and BBS-NJ 16SrI-E strains belonged to a closely related lineage, while BBS-NJ 16SrIX-E strains and JunWB strains represented two distinct lineages. Single nucleotide polymorphisms (SNPs) analyses of rp and secY gene sequences further revealed that no specific rp gene SNPs and only two specific secY gene SNPS were present between BBS-NJ 16SrI-E strains and BBS3-AR. In contrast, BBS-NJ 16SrIX-E strains/clones had 15 consensus rp SNPs and 28 consensus secY SNPs that separated them from JunWB strains/clones. For the first time, two distinct phytoplasmas that cause BBS-disease in the U.S. was revealed.

译文

:采用巢式PCR分析法检测了2010年和2011年收集的127株表现出典型或部分蓝莓特技(BBS)综合征的蓝莓植物中的植物浆原体,这些植物主要分布于美国新泽西州的两个县中的11个商业农场。表现出典型特技综合症的九十株植物检测出的植物浆体感染呈阳性。限制性片段长度多态性(RFLP)分析表明,两个不同的植物质原体与BBS病害的植物有关。之前鉴定出的约95%的植物原质与BBS植物原虫菌株BBS3-AR(亚组16SrI-E)和BBS1-MI(未鉴定)密切相关,而检出的植物原质中有4.4%属于木豆巫婆扫帚植物原质组。 (16SrIX)。克隆的16S rDNA的序列和系统发育分析进一步表明,亚组16SrI-E相关的植物质浆体代表16SrI-E参考菌株BBS3-AR的变体,而16SrIX相关的植物质体与杜松witch帚(JunWB)植物质体(16SrIX -E),代表16SrIX-E变体。核糖体蛋白(rp)和secY基因为基础的系统发育研究表明BBS3-AR和BBS-NJ 16SrI-E菌株属于密切相关的血统,而BBS-NJ 16SrIX-E菌株和JunWB菌株则代表两个不同的血统。 rp和secY基因序列的单核苷酸多态性(SNPs)分析进一步表明,在BBS-NJ 16SrI-E菌株和BBS3-AR之间不存在特定的rp基因SNP,而仅存在两个特定的secY基因SNPS。相反,BBS-NJ 16SrIX-E菌株/克隆具有15个共有rp SNP和28个共有secY SNP,将它们与JunWB菌株/克隆分开。首次揭示了在美国引起BBS疾病的两种不同的植物原质。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录