Diets containing unheated casein (CD; control) or a casein-glucose mixture (CGD) previously heated at 140 degrees for 2 h were fed to two groups of young rats for 21 d. Differences in body weight, feed consumption, thymus, and spleen growth, protein metabolism and in vivo immune response were then determined. For this last experiment, animals were inoculated with sheep erythrocytes (SRBC) on day 15 to provide an immunological challenge. No changes were seen in body weight, feed consumption or feed conversion ratios. Neither were significant differences found in spleen weight, protein content, DNA content, DNase (EC 3.1.4.6) activity or lymphocyte count, suggesting that spleen cell growth remained similar in all the animals studied. The CGD induced marked increases in thymus DNA content whilst the protein:DNA ratio became lower. Spleen RNA content was similar in all rats, but thymus RNA content was 29% lower in the CGD group, although this difference did not reach statistical significance. This fact might be a consequence of the low RNase (EC 2.7.7.16) activity and RNase:RNA ratios in the thymus glands of CGD-fed animals. Further, the number of splenic plasma cells secreting anti-SRBC antibodies (direct plaque-forming cells) was significantly decreased in the same group. It might be concluded that both diets are adequate for rat growth and that the differences observed in the thymus of CGD-fed rats may be directed towards preserving tissue function. Nevertheless, the CGD did cause immunological disturbances affecting the humoral immune response.

译文

将含有未加热的酪蛋白(CD;对照)或酪蛋白-葡萄糖混合物(CGD)的先前已在140度下加热2小时的饮食喂食到两组幼鼠,持续21天。然后确定体重,饲料消耗,胸腺和脾脏生长,蛋白质代谢和体内免疫反应的差异。对于最后一个实验,在第15天用羊红细胞(SRBC)接种动物以提供免疫学攻击。体重,饲料消耗或饲料转化率未见变化。脾脏重量,蛋白质含量,DNA含量,DNase(EC 3.1.4.6)活性或淋巴细胞计数均未发现显着差异,表明脾脏细胞生长在所有研究的动物中均相似。 CGD诱导胸腺DNA含量显着增加,而蛋白质:DNA比例降低。在所有大鼠中,脾脏RNA含量均相似,但CGD组的胸腺RNA含量低29%,尽管这种差异并未达到统计学意义。此事实可能是CGD喂养的动物的胸腺中RNase(EC 2.7.7.16)活性低和RNase:RNA比低的结果。此外,同一组中分泌抗SRBC抗体的脾浆细胞(直接噬菌斑形成细胞)数量明显减少。可以得出结论,两种饮食都足以使大鼠生长,并且在CGD喂养的大鼠的胸腺中观察到的差异可能是为了保持组织功能。尽管如此,CGD确实会引起影响体液免疫反应的免疫学紊乱。

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