BACKGROUND:Obesity is associated with gut microbiota dysbiosis, disrupted intestinal barrier and chronic inflammation. Given the high and increasing prevalence of obesity worldwide, anti-obesity treatments that are safe, effective and widely available would be beneficial. We examined whether the medicinal mushroom Antrodia cinnamomea may reduce obesity in mice fed with a high-fat diet (HFD). METHODS:Male C57BL/6J mice were fed a HFD for 8 weeks to induce obesity and chronic inflammation. The mice were treated with a water extract of A. cinnamomea (WEAC), and body weight, fat accumulation, inflammation markers, insulin sensitivity and the gut microbiota were monitored. RESULTS:After 8 weeks, the mean body weight of HFD-fed mice was 39.8±1.2 g compared with 35.8±1.3 g for the HFD+1% WEAC group, corresponding to a reduction of 4 g or 10% of body weight (P<0.0001). WEAC supplementation reduced fat accumulation and serum triglycerides in a statistically significant manner in HFD-fed mice. WEAC also reversed the effects of HFD on inflammation markers (interleukin-1β, interleukin-6, tumor necrosis factor-α), insulin resistance and adipokine production (leptin and adiponectin). Notably, WEAC increased the expression of intestinal tight junctions (zonula occludens-1 and occludin) and antimicrobial proteins (Reg3g and lysozyme C) in the small intestine, leading to reduced blood endotoxemia. Finally, WEAC modulated the composition of the gut microbiota, reducing the Firmicutes/Bacteroidetes ratio and increasing the level of Akkermansia muciniphila and other bacterial species associated with anti-inflammatory properties. CONCLUSIONS:Supplementation with A. cinnamomea produces anti-obesogenic, anti-inflammatory and antidiabetic effects in HFD-fed mice by maintaining intestinal integrity and modulating the gut microbiota.

译文

背景:肥胖症与肠道菌群失调,肠壁屏障破坏和慢性炎症有关。鉴于世界范围内肥胖症的发病率不断上升,安全,有效和广泛使用的抗肥胖症治疗方法将是有益的。我们检查了药用蘑菇牛樟芝是否可以减少高脂饮食(HFD)喂养的小鼠的肥胖。
方法:雄性C57BL / 6J小鼠接受HFD喂养8周,以诱导肥胖和慢性炎症。用肉桂曲霉(WEAC)的水提取物治疗小鼠,并监测体重,脂肪堆积,炎症标志物,胰岛素敏感性和肠道菌群。
结果:8周后,喂食HFD的小鼠的平均体重为39.8±1.2 g,而喂食1%WEAC的HFD组的平均体重为35.8±1.3 g,相当于减少了4 g或体重的10%(P < 0.0001)。在HFD喂养的小鼠中,WEAC补充剂以统计学上显着的方式减少了脂肪积累和血清甘油三酯。 WEAC还逆转了HFD对炎症标志物(白介素-1β,白介素-6,肿瘤坏死因子-α),胰岛素抵抗和脂肪因子产生(瘦素和脂联素)的影响。值得注意的是,WEAC增加了小肠中肠紧密连接(小肠闭合带1和闭合蛋白)和抗菌蛋白(Reg3g和溶菌酶C)的表达,从而降低了血液内毒素血症。最后,WEAC调节了肠道菌群的组成,降低了Firmicutes / Bacteroidetes的比例,并增加了Akkermansia muciniphila和其他与抗炎特性相关的细菌的水平。
结论:补充肉桂曲霉可通过维持肠道完整性和调节肠道菌群,在喂食HFD的小鼠中产生抗肥胖,抗炎和抗糖尿病作用。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录