Bisabololoxide A (BSBO), main constituents in German chamomile extract, is responsible for antipruritic effect. In previous study, the incubation with 30-100 μM BSBO for 24 h exerted cytotoxic and proapoptotic effects on rat thymocytes. To further characterize BSBO cytotoxicity, the effect on the cells suffering from calcium overload by calcium ionophore A23187 was examined. A23187 induced Ca(2+) -dependent cell death. Contrary to our expectation, 1-10 μM BSBO inhibited A23187-induced increase in cell lethality of rat thymocytes. BSBO attenuated A23187-induced increases in populations of shrunken living cells, phosphatidylserine-exposed living cells, and dead cells, without affecting the increase in intracellular Ca(2+) concentration and the Ca(2+) -dependent hyperpolarization. The effect of BSBO on A23187-treated cells may be unique because the activation of Ca(2+) -dependent K(+) channels is required for cell shrinkage, externalization of phosphatidylserine, and cell death in some cells. The cell death induced by A23187 was not inhibited by Z-VAD-FMK, a pan-inhibitor of caspases. Thus, the cell death may be a necrosis with some features observed during an early stage of apoptosis. These results suggest that BSBO at low micromolar concentrations is cytoprotective against calcium overload.

译文

德国洋甘菊提取物中的主要成分:双酚A(BSBO)具有止痒作用。在以前的研究中,用30-100μMBSBO孵育24h会对大鼠胸腺细胞产生细胞毒性和促凋亡作用。为了进一步表征BSBO的细胞毒性,研究了钙离子载体A23187对钙超负荷细胞的作用。 A23187诱导Ca(2)依赖性细胞死亡。与我们的预期相反,1-10μMBSBO抑制了A23187诱导的大鼠胸腺细胞杀伤力的增加。 BSBO衰减收缩的活细胞,磷脂酰丝氨酸暴露的活细胞和死细胞的人口中的A23187诱导的增加,而不影响细胞内Ca(2)浓度和Ca(2)依赖性超极化的增加。 BSBO对A23187处理的细胞的影响可能是独特的,因为激活Ca(2)依赖的K()通道对于细胞收缩,磷脂酰丝氨酸的外在化和某些细胞的细胞死亡是必需的。胱天蛋白酶的泛抑制剂Z-VAD-FMK未抑制由A23187诱导的细胞死亡。因此,细胞死亡可能是坏死,在细胞凋亡的早期阶段观察到某些特征。这些结果表明,低微摩尔浓度的BSBO对钙超载具有细胞保护作用。

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