Herein, corn silk extract and its flavonoids were used to inhibit the formation of Nε-carboxymethyllysine (CML) in a casein glucose-fatty acid model system. Under these optimum extraction conditions, nine major flavonoids were identified and quantified by HPLC-MS/MS. The percent inhibition of CML formation by corn silk extract was 76.57%. The inhibitory mechanism of corn silk extract toward CML formation was further investigated by examining the trapping of glyoxal/methyl glyoxal by the major flavonoids (5 mM) using HPLC-ESI-MS, and mono-, di-, and tri-adducts were found for some flavonoid compounds. The antioxidant activity of the corn silk extract was evaluated by the DPPH and ABTS assays. The scavenging activity of the corn silk extract for DPPH and ABTS was 84.38% and 89.11%, respectively. The results suggested that corn silk extract inhibited CML formation through glyoxal/methyl glyoxal scavenging or by its antioxidant activity attributed to its flavonoid content.

译文

:在酪蛋白葡萄糖-脂肪酸模型系统中,玉米丝提取物及其类黄酮用于抑制Nε-羧甲基赖氨酸(CML)的形成。在这些最佳提取条件下,通过HPLC-MS / MS鉴定并定量了九种主要类黄酮。玉米丝提取物对CML形成的抑制百分比为76.57%。通过使用HPLC-ESI-MS检测主要黄酮(5mM)对乙二醛/甲基乙二醛的捕集,进一步研究了玉米丝提取物对CML形成的抑制机理,发现了单加合物,双加合物和三加合物一些类黄酮化合物。玉米丝提取物的抗氧化活性通过DPPH和ABTS分析进行评估。玉米丝提取物对DPPH和ABTS的清除活性分别为84.38%和89.11%。结果表明玉米丝提取物通过乙二醛/甲基乙二醛清除或由于其类黄酮含量引起的抗氧化活性抑制了CML的形成。

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