Lindera obtusiloba Blume, a native plant of East Asia, has traditionally been used as a folk medicine for liver disease. We studied the in vitro antioxidant and in vivo hepatoprotective activities of a 70% ethanolic extract of L. obtusiloba (LOE) containing 62.9% quercitrin and 22.0% afzelin. LOE prevented tert-butyl hydroperoxide (t-BHP)-induced oxidative damage in HepG2 cells. Along with its high antioxidant potency in vitro, our animal study confirmed that pretreatment with LOE (500 or 2000 mg/kg) for 7 days prior to a single dose of t-BHP (i.p.: 0.5 mmol/kg) significantly lowered the serum levels of alanine and aspartate aminotransferases. In addition, glutathione levels were increased in the liver, and lipid peroxidation levels were decreased in a dose-dependent manner. The histopathological examinations of rat livers showed that LOE significantly reduced the incidence of liver lesions induced by t-BHP. Therefore, we concluded that LOE has merit as a potent candidate to protect the liver against oxidative damage.

译文

:Lindera obtusiloba Blume是东亚的本地植物,传统上一直用作肝病的民间药物。我们研究了含有62.9%槲皮苷和22.0%afzelin的70%Obtusiloba乳杆菌(LOE)乙醇提取物的体外抗氧化剂和体内保肝活性。 LOE阻止了叔丁基氢过氧化物(t-BHP)诱导的HepG2细胞氧化损伤。除了在体外具有很高的抗氧化能力外,我们的动物研究还证实,在单剂量t-BHP(ip:0.5 mmol / kg)之前,用LOE(500或2000 mg / kg)预处理7天可显着降低血清水平丙氨酸和天冬氨酸转氨酶。此外,肝脏中的谷胱甘肽水平升高,脂质过氧化水平以剂量依赖性方式降低。对大鼠肝脏的组织病理学检查显示,LOE显着降低了t-BHP诱导的肝脏病变的发生率。因此,我们得出结论,LOE作为保护肝脏免于氧化损伤的有效候选者而值得。

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