We examined by immunoblot analysis the serum immunoglobulin G antibody response to EDTA-extracted surface proteins of Clostridium difficile in 16 patients with antibiotic-associated diarrhea. For each patient, paired serum samples were tested against proteins of the infecting strain and of a collection strain (C253) known to belong to the electrophoretic group 2 pattern. Eight patients, all harboring group 2 C. difficile strains, exhibited responses to the proteins of the infecting strain; six patients showed increases in the level of antibodies between acute-phase and convalescent-phase sera. A great variability in the antigens recognized was found; however, seven patients possessed antibodies directed against an antigen of about 35 kilodaltons, corresponding to the major protein of group 2 strains. The sera of these seven patients cross-reacted also with the 35-kilodalton and other proteins of strain C253. Our data show that C. difficile proteins other than toxins can elicit an immune response in patients with C. difficile-associated disease; in this group of patients, the major surface protein of the group 2 strains was the antigen most often recognized.

译文

:我们通过免疫印迹分析检查了16例抗生素相关性腹泻患者的血清免疫球蛋白G抗体对EDTA提取的艰难梭菌表面蛋白的反应。对于每位患者,测试成对的血清样品中的感染菌株和收集菌株(C253)的蛋白质,这些蛋白质已知属于电泳2组模式。八名患者均携带第2组艰难梭菌菌株,对感染菌株的蛋白质表现出反应。 6名患者显示急性期和恢复期血清之间的抗体水平升高。发现公认的抗原有很大的变异性。然而,有7名患者拥有针对约35道尔顿的抗原的抗体,该抗原对应于第2组毒株的主要蛋白质。这7名患者的血清也与35-千屈顿和C253菌株的其他蛋白发生交叉反应。我们的数据表明,除毒素外,艰难梭菌蛋白还可以在艰难梭菌相关疾病患者中引发免疫反应。在这组患者中,第2组菌株的主要表面蛋白是最常识别的抗原。

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