Hoxa-5 is a homeobox gene that is highly expressed in the developing mouse lung. However, little is known about the molecular mechanisms controlling expression. We characterized the ontogeny of Hoxa-5 gene and protein expressions during lung development and then studied the cell-specific effects of retinoic acid (RA) on Hoxa-5 mRNA in fetal lung fibroblasts and MLE-12 mouse lung epithelial cells. Strong but constant Hoxa-5 gene and protein expressions were detected from mouse lung on embryonic day 13.5 to postnatal day 2. At baseline, the gene was strongly expressed in the fibroblasts of day 17.5 fetal mouse lungs. A very weak but reproducible expression was present in the MLE-12 cells. RA stimulated gene expression in both cell types in a time- and dose-dependent manner. Peak expression occurred much later in the MLE-12 cells compared with that in fibroblasts. Cycloheximide and actinomycin D treatment studies suggested that the differences in RA effect on each cell type may involve the presence of a repressor that can be overcome by RA.

译文

:Hoxa-5是一个同源盒基因,在发育中的小鼠肺中高度表达。但是,关于控制表达的分子机制知之甚少。我们表征了肺发育过程中Hoxa-5基因和蛋白质表达的存在,然后研究了维甲酸(RA)对胎儿肺成纤维细胞和MLE-12小鼠肺上皮细胞中Hoxa-5 mRNA的细胞特异性作用。在胚胎第13.5天至出生后第2天从小鼠肺中检测到强而恒定的Hoxa-5基因和蛋白质表达。在基线时,该基因在17.5天胎儿小鼠肺的成纤维细胞中强烈表达。 MLE-12细胞中存在非常弱但可重现的表达。 RA以时间和剂量依赖性方式刺激两种细胞类型中的基因表达。与成纤维细胞相比,MLE-12细胞的峰值表达要晚得多。环己酰亚胺和放线菌素D的治疗研究表明,RA对每种细胞类型的作用差异可能涉及存在可以被RA克服的阻遏物。

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