The oxygenated hypolimnion accounts for a volumetrically significant part of the global freshwater systems. Previous studies have proposed the presence of hypolimnion-specific bacterioplankton lineages that are distinct from those inhabiting the epilimnion. To date, however, no consensus exists regarding their ubiquity and abundance, which is necessary to evaluate their ecological importance. The present study investigated the bacterioplankton community in the oxygenated hypolimnia of 10 deep freshwater lakes. Despite the broad geochemical characteristics of the lakes, 16S rRNA gene sequencing demonstrated that the communities in the oxygenated hypolimnia were distinct from those in the epilimnia and identified several predominant lineages inhabiting multiple lakes. Catalyzed reporter deposition fluorescence in situ hybridization revealed that abundant hypolimnion-specific lineages, CL500-11 (Chloroflexi), CL500-3, CL500-37, CL500-15 (Planctomycetes) and Marine Group I (Thaumarchaeota), together accounted for 1.5-32.9% of all bacterioplankton in the hypolimnion of the lakes. Furthermore, an analysis of single-nucleotide variation in the partial 16S rRNA gene sequence (oligotyping) suggested the presence of different sub-populations between lakes and water layers among the lineages occurring in the entire water layer (for example, acI-B1 and acI-A7). Collectively, these results provide the first comprehensive overview of the bacterioplankton community in the oxygenated hypolimnion of deep freshwater lakes.

译文

含氧的低水离子在全球淡水系统中占很大一部分。先前的研究已经提出,存在特定于hypolimnion的浮游细菌谱系,这些谱系与居住在epilimnion的细菌谱系不同。然而,迄今为止,关于它们的普遍性和丰富性尚无共识,这是评估其生态重要性所必需的。本研究调查了10个深淡水湖的含氧低泡中的浮游细菌群落。尽管湖泊具有广泛的地球化学特征,但16S rRNA基因测序表明,含氧的低水草中的群落与上水草中的群落不同,并确定了居住在多个湖泊中的几个主要谱系。催化的报告分子沉积荧光原位杂交显示,丰富的hypolinion特异性谱系,CL500-11 (Chloroflexi),CL500-3,CL500-37,CL500-15 (planctoycetes) 和海洋I类 (Thaumarchaeota),占所有浮游细菌的1.5-32.9% 在湖泊的hypolinion中。此外,对部分16 srrna基因序列中的单核苷酸变异的分析 (寡核苷酸分型) 表明,在整个水层中发生的谱系 (例如,acI-B1和acI-A7) 中,湖泊和水层之间存在不同的亚群。总的来说,这些结果首次全面概述了深淡水湖含氧的低水量中的浮游细菌群落。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录