The development of more productive strains of microorganisms and processes that increase enzyme levels can contribute to the economically efficient production of second generation ethanol. To this end, cellulases and xylanases were produced with the S1M29 mutant strain of Penicillium echinulatum, using different concentrations of cellulose (20, 40, and 60 g L(-1)) in batch and fed-batch processes. The highest activities of FPase (8.3 U mL(-1)), endoglucanases (37.3 U mL(-1)), and xylanases (177 U mL(-1)) were obtained in fed-batch cultivation with 40 g L(-1) of cellulose. The P. echinulatum enzymatic broth and the commercial enzyme Cellic CTec2 were tested for hydrolysis of pretreated sugar cane bagasse. Maximum concentrations of glucose and xylose were achieved after 72 h of hydrolysis. Glucose yields of 28.0% and 27.0% were obtained using the P. echinulatum enzymatic extract and Cellic CTec2, respectively.

译文

开发更具生产力的微生物菌株和提高酶水平的工艺可以有助于经济高效地生产第二代乙醇。为此,在分批和补料分批过程中,使用不同浓度的纤维素 (20、40和60g L(-1)),用棘青霉的S1M29突变菌株生产纤维素酶和木聚糖酶。在用40g L(-1) 纤维素补料分批培养中获得最高的FPase (8.3 U mL(-1)) 、内切葡聚糖酶 (37.3 U mL(-1)) 和木聚糖酶 (177 U mL(-1)) 活性。测试了echinulatum酶促肉汤和商业酶Cellic CTec2对预处理的甘蔗渣的水解作用。水解72小时后达到最大浓度的葡萄糖和木糖。分别使用棘皮草酶提取物和Cellic CTec2获得28.0% 和27.0% 的葡萄糖产量。

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