Complete nucleotide sequence of the tissue culture-adapted ATCC-Wa strain of human rotavirus NSP4 was determined. Sequence analysis detected two alternate forms of the gene with a nucleotide difference at position 331 (A or G) in the coding sequence (NSP4-A or NSP4-G) leading to a change from neutral glutamine97 in NSP4-A to a positively charged arginine97 in NSP4-G originating from the same ATCC-Wa preparation. In addition to this, both forms of ATCC-Wa NSP4 revealed three mutations at nucleotide positions 88 (T to C), 142 (C to T) and 572 (G to A), when compared to the previously reported NSP4 sequence from virulent Wa strain. The former two mutations were in the coding sequence and resulted in a leucine16 to serine16 and a proline34 to leucine34 change, while the third mutation was in the 3' non-coding region of the gene. The two amino acid changes may contribute to the 'tissue culture-adaptation' of ATCC-Wa strain. The ATCC-Wa NSP4 sequence was found to differ from the previously reported NSP4 sequence of attenuated Wa strain by lacking a mutation at 133 (T to C), though the mutations at 88 and 142 were present in both strains. Furthermore, comparison of deduced amino acid sequence of NSP4 from human, bovine, porcine and simian rotavirus strains identified a seven-residue (positions 135-141) inter-species variable domain in its C-terminal region.

译文

确定了人类轮状病毒NSP4的组织培养适应的atcc-wa株的完整核苷酸序列。序列分析检测到在编码序列 (NSP4-A或NSP4-G) 中位置331 (a或G) 处具有核苷酸差异的基因的两种替代形式,导致从NSP4-A的中性谷氨酰胺97变为源自相同atcc-wa制剂的NSP4-G中的带正电荷的精氨酸97。除此之外,当与先前报道的来自强毒Wa株的NSP4序列相比时,两种形式的atcc-wa NSP4在核苷酸位置88 (T至C) 、142 (C至T) 和572 (G至A) 处揭示了三个突变。前两个突变在编码序列中,导致亮氨酸16到丝氨酸16和脯氨酸34到亮氨酸34的变化,而第三个突变在基因的3' 非编码区。这两个氨基酸的变化可能有助于atcc-wa菌株的 “组织培养适应”。发现atcc-wa NSP4序列与先前报道的减毒Wa菌株的NSP4序列不同,因为在133 (T至C) 处没有突变,尽管在88和142处的突变存在于两个菌株中。此外,从人,牛,猪和猿猴轮状病毒株中推导的NSP4氨基酸序列的比较确定了其C末端区域中的七个残基 (位置135-141) 种间可变结构域。

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