pH 5.9 is optimal for tobacco pollen tube growth in suspension culture. Little pH changes of sugar-mineral medium result from the release of surface-linked compounds from pollen grains. Germination and pollen tube growth bring about a progressive medium acidification resulting in total growth inhibition. An increase of the buffering capacity of the culture medium enhances pollen tube growth. When the pH was kept near the optimum by 25 mM MES-KOH buffer, pollen tubes grew for 4 days and in the presence of casein hydrolysate they reached a length of up to about 4 cm. The growth related acidification of the medium is independent of the presence of mineral cations and is not due to the hydration of respiratory CO(2). It is suggested that it is brought about by proton secretion from pollen tubes.

译文

pH 5.9对于悬浮培养中的烟草花粉管生长是最佳的。糖矿物培养基的ph值变化很小,这是由于花粉粒中表面连接的化合物的释放所致。发芽和花粉管生长会导致培养基酸化,从而导致总生长抑制。培养基缓冲能力的增加可增强花粉管的生长。当通过25mmmes-KOH缓冲液将pH保持在最佳值附近时,花粉管生长4天,并且在酪蛋白水解产物存在下,它们达到高达约4厘米的长度。与生长相关的培养基酸化与矿物阳离子的存在无关,而不是由于呼吸CO(2) 的水合作用。建议它是由花粉管的质子分泌引起的。

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