Alveolar type II cells were isolated from fetal mouse lung by differential adherence and obtained in monolayer culture. Cultures display a high degree of purity as shown by histochemical and immunocytochemical staining procedures. Seventy-five percent of cells stained positive with specific anti-lavage serum mouse (SALS-M), an antiserum specific for (pre)alveolar type II cells of the mouse, and osmiophilic bodies were present in 82% of cells. These and other characteristics of type II cells in culture correspond to those of alveolar type II cells in fetal mouse lung. The pattern of reactivity of these cells with various anti-cytokeratin antibodies is described, and we show that, in contrast to rat type II cells, they do not exhibit alkaline phosphatase activity. Identity of the type II cell cultures was shown by their specific phospholipid composition and surfactant protein A (SP-A) content. The fetal alveolar type II cells in culture were found to synthesize and express class I but not class II major histocompatibility complex (MHC) antigens. The possibility to culture fetal alveolar type II cells of the mouse and the availability of genetically well-defined inbred and transgenic mouse strains opens ways to study the genetics of type II cell differentiation and function. Also, the in vitro availability of alveolar type II cells, the progenitor cells of mouse lung tumors, will enable us to study in vitro several of the processes involved in lung tumorigenesis in the mouse.

译文

通过差异粘附从胎鼠肺中分离出II型肺泡细胞,并在单层培养中获得。如组织化学和免疫细胞化学染色程序所示,培养物显示出高度的纯度。用特异性抗灌洗液血清小鼠 (sars-M) 染色阳性的5% 个细胞,该血清对小鼠的 (前) 肺泡II型细胞具有特异性的抗血清,和嗜渗体体存在于细胞82% 中。培养的II型细胞的这些和其他特征对应于胎鼠肺中II型肺泡细胞的那些特征。描述了这些细胞与各种抗细胞角蛋白抗体的反应性模式,并且我们表明,与大鼠II型细胞相反,它们不显示碱性磷酸酶活性。II型细胞培养物的身份通过其特定的磷脂组成和表面活性剂蛋白A (sp-a) 含量显示。发现培养物中的胎儿肺泡II型细胞合成并表达I类但不表达II类主要组织相容性复合物 (MHC) 抗原培养小鼠胎儿肺泡II型细胞的可能性以及遗传明确的近交和转基因小鼠品系的可用性为研究II型细胞分化和功能的遗传学开辟了途径。此外,肺泡II型细胞 (小鼠肺肿瘤的祖细胞) 的体外可用性将使我们能够在体外研究与小鼠肺肿瘤发生有关的几个过程。

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