Colony-forming cells (CFC) and long-term culture-initiating cells (LTC-IC) include a spectrum of progenitor types whose potential contributions to the haemopoietic recovery seen in patients transplanted with mobilized peripheral blood progenitor cells (PBPC) remains unclear. We evaluated both the number and cycling status of the circulating LTC-IC and CFC harvested from 12 patients treated with chemotherapy and G-CSF using a modified 6-week LTC-IC assay. The frequency of the LTC-IC and CFC in the mobilized PB samples were increased 45- and 750-fold, respectively. Interestingly, comparison of these values for PB samples, taken just prior to the start of the leukapheresis, with the progenitor content of the 3 h harvest, showed that, on average, the leukapheresis product contained 19 times more LTC-IC (P < 0.01) than had been detectable in the entire blood volume of the patients at the start of the collection, whereas the number of CFC collected was approximately the same as the number in the initial circulating pool of PBPC. Cycling studies showed many of the LTC-IC in the apheresis collections to be proliferating although not more so than in the steady-state marrow LTC-IC compartment (i.e. per cent kill of mobilized LTC-IC after 16 h in 3H-Tdr = 70 +/- 8%, n = 9). On the other hand, the majority of the CFC in the apheresis collections were initially quiescent (per cent kill after 16 h in 3H-Tdr = 37 +/- 6%, n = 12). These findings demonstrate the rapidity with which a primitive subset of LTC-IC may enter the circulation during the early phase of rebound haemopoiesis induced by chemotherapy plus G-CSF and provide evidence of differences in the mechanisms regulating LTC-IC and CFC mobilization.

译文

集落形成细胞 (CFC) 和长期培养起始细胞 (LTC-IC) 包括一系列祖细胞类型,其对移植有动员的外周血祖细胞 (PBPC) 的患者的造血恢复的潜在贡献尚不清楚。我们使用改良的6周LTC测定法评估了从接受化学疗法和g-csf治疗的12例患者中收集的循环LTC-IC和CFC的数量和循环状态。动员的PB样品中LTC-IC和CFC的频率分别增加了45倍和750倍。有趣的是,将白细胞分离术开始之前的PB样品的这些值与3小时收获的祖细胞含量进行比较,结果表明,平均而言,白细胞分离产物含有的LTC-IC (P < 0.01) 比在收集开始时在患者的全部血容量中检测到的LTC多19倍,而收集的CFC的数量与PBPC的初始循环池中的数量大致相同。循环研究表明,单采收集中的许多LTC-IC正在增殖,尽管并不比稳态骨髓LTC-IC区室中的增殖更多 (即在3H-Tdr = 70 +/- 8% 中,16小时后动员的LTC-IC的杀死率,n = 9)。另一方面,单采收集中的大多数CFC最初是静止的 (在3H-Tdr = 37/- 6%,n = 12中,在16小时后杀死 %)。这些发现证明了LTC-IC的原始子集在化疗加g-csf诱导的反弹造血的早期阶段可能进入循环的速度,并提供了调节LTC-IC和CFC动员机制差异的证据。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录