We performed a prospective study to compare the Etest and the microdilution method (NCCLS guidelines) for determining the MICs of fluconazole, itraconazole, flucytosine and amphotericin B for 35 strains of Cryptococcus neoformans. For the microdilution method (MDM) RPMI 1640 medium with 2% glucose was used for fluconazole, itraconazole and flucytosine, and Antibiotic Medium 3 for amphotericin B. For the Etest, RPMI 1640 medium with 2% glucose and solidified with 1.5% agar was used for the four antifungal agents. Amphotericin B was also tested on Antibiotic Medium 3 solidified with 1.5% agar. Fluconazole and flucytosine MICs by the Etest showed good correlation with the broth MDM (81.1 and 89.2% agreement within two dilutions, respectively). With the tested population of itraconazole- and amphotericin B-susceptible isolates, the MIC agreement for itraconazole was 54%; amphotericin B showed the lowest agreement (8.1% on Antibiotic Medium 3 and 13.5% on RPMI).

译文

我们进行了一项前瞻性研究,以比较Etest和微量稀释法 (NCCLS指南) 来确定35株新型隐球菌的氟康唑,伊曲康唑,氟胞嘧啶和两性霉素b的mic。对于微量稀释法 (MDM),将含有2% 葡萄糖的RPMI 1640培养基用于氟康唑,伊曲康唑和氟胞嘧啶,将抗生素培养基3用于两性霉素b。对于Etest,将具有2% 葡萄糖并用1.5% 琼脂固化的RPMI 1640培养基用于四种抗真菌剂。还在用1.5% 琼脂固化的抗生素培养基3上测试两性霉素b。Etest的氟康唑和氟胞嘧啶mic与肉汤MDM显示出良好的相关性 (分别在两个稀释液中81.1和89.2% 一致)。在测试的伊曲康唑和两性霉素b敏感分离株的群体中,伊曲康唑的MIC一致性为54%; 两性霉素b显示出最低的一致性 (在抗生素培养基3上8.1%,在RPMI上13.5%)。

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