Adipose tissue-derived stem/progenitor cells (ASCs) have attracted attention as a cell source that replaces marrow stromal cells (MSCs); ASCs may thus have applications in both regenerative medicine and cell transplantation. These medical treatments, however, require a high-quality supply of human ASCs. Therefore, the cryopreservation methods have been improved by changing a component of a cryopreservation medium. Sericin, a protein hydrolysate (with an average molecular weight of 30 kDa) is very rich in serine. The viability and the adipogenic/osteogenic potential of human ASCs were tested after freezing in a cryopreservation medium containing sericin. After thawing, the viability of the human ASCs frozen in the cryopreservation medium was found to be more than 95%. The proliferation rate of human ASCs frozen in CELLBANKER 2, and DMEM/Ham's F-12 medium (serum free) + 10% DMSO, 0.1 mol/L maltose, and 1% sericin was higher than that of the cells frozen in the maintenance medium + 10% DMSO. The adipogenic/osteogenic differentiation capabilities of frozen human ASCs were examined by Oil Red O staining/Von Kossa's method. The human ASCs were frozen using CELLBANKER 2, and DMEM/Ham's F-12 medium (serum free) + 10% DMSO, 0.1 mol/L maltose, and 1% sericin were positive. In conclusion, the cryopreservation medium containing sericin is therefore considered to have a beneficial effect on freezing human ASCs. This serum-free cryopreservation medium should be widely used in regenerative medicine, cell transplantation, and biological research.

译文

脂肪组织来源的干/祖细胞 (ASCs) 作为替代骨髓基质细胞 (MSCs) 的细胞来源引起了人们的关注; 因此,ASCs可能在再生医学和细胞移植中都有应用。然而,这些医疗需要高质量的人类asc供应。因此,通过改变低温保存介质的成分,对低温保存方法进行了改进。丝胶,一种蛋白质水解物 (平均分子量为30 kDa),丝氨酸含量非常丰富。在含有丝胶蛋白的冷冻保存培养基中冷冻后,测试了人asc的活力和成脂/成骨潜力。解冻后,发现冷冻在冷冻培养基中的人asc的活力大于95%。在CELLBANKER 2和DMEM/Ham's F-12培养基 (无血清) 10% DMSO,0.1 mol/L麦芽糖和1% 丝胶中冷冻的人ASCs的增殖速率高于在维持培养基10% DMSO中冷冻的细胞的增殖速率。通过油红O染色/Von Kossa方法检查了冷冻人asc的成脂/成骨分化能力。使用CELLBANKER 2冷冻人asc,并且DMEM/Ham's F-12培养基 (无血清) + 10% DMSO,0.1 mol/L麦芽糖和1% 丝胶为阳性。总之,因此,含有丝胶蛋白的冷冻培养基被认为对冷冻人asc具有有益的作用。这种无血清低温保存培养基应广泛应用于再生医学、细胞移植和生物学研究。

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