Vitrification is considered as the most promising method for long-term storage of tissues and organs. An effective way to reduce the accompanied cryoprotectant (CPA) toxicity, during CPA addition/removal, is to operate at low temperatures. The permeation process of CPA into/out of biomaterials is affected by the viscosity of CPA solution, especially at low temperatures. The objective of the present study is to measure the viscosity of the ternary solution, dimethyl sulfoxide (Me2SO)/water/sodium chloride (NaCl), at low temperatures and in a wide range of concentrations. A rotary viscometer coupled with a low temperature thermostat bath was used. The measurement was carried out at temperatures from -10 to -50°C. The highest mass fraction of Me2SO was 75% (w/w) and the lowest mass fraction of Me2SO was the value that kept the solution unfrozen at the measurement temperature. The concentration of NaCl was kept as a constant [0.85% (w/w), the normal salt content of extracellular fluids]. The Williams-Landel-Ferry (WLF) model was employed to fit the obtained viscosity data. As an example, the effect of solution viscosity on modeling the permeation of Me2SO into articular cartilage was qualitatively analyzed.

译文

玻璃化被认为是组织和器官长期储存的最有前途的方法。在添加/去除CPA期间,降低伴随的冷冻保护剂 (CPA) 毒性的有效方法是在低温下运行。CPA进入/离开生物材料的渗透过程受CPA溶液粘度的影响,尤其是在低温下。本研究的目的是在低温和较宽的浓度范围内测量三元溶液二甲基亚砜 (Me2SO)/水/氯化钠 (NaCl) 的粘度。使用了旋转粘度计和低温恒温浴。测量是在-10至-50 °C的温度下进行的。Me2SO的最高质量分数是75% (w/w),Me2SO的最低质量分数是使溶液在测量温度下保持解冻的值。NaCl的浓度保持恒定 [0.85% (w/w),即细胞外液的正常盐含量]。采用Williams-Landel-Ferry (WLF) 模型拟合获得的粘度数据。例如,定性分析了溶液粘度对模拟Me2SO渗透到关节软骨中的影响。

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