In our centre, embryos are judged to have survived cryopreservation if at least half of the initial number of blastomeres remain intact. Therefore both fully intact and partially damaged embryos are transferred. The aim of this study was to investigate the viability of partially damaged human embryos after cryopreservation. We retrospectively analysed the implantation and in-vivo development of embryos which were either fully intact or had lost some blastomeres after cryopreservation. Oocytes were collected following stimulation with the gonadotrophin-releasing hormone (GnRH)-agonist Buserelin and human menopausal gonadotrophin. Supernumerary multicellular embryos with not more than 20% of their volume filled with anucleate fragments were frozen on day 2 or day 3 of the cycle using a slow cooling procedure with dimethylsulphoxide as the cryoprotectant. Following slow thawing, 431 fully intact embryos were transferred in 314 embryo transfer procedures and 488 partially damaged embryos were transferred in 327 such procedures. The percentage of gestational sacs with fetal heartbeat obtained after transfer of fully intact embryos was almost three times higher than that after transfer of partially damaged embryos (11.4 versus 3.5%). Forty-five children (birth rate 10% per embryo transfer) were born after transfer of fully intact embryos and 14 after transfer of embryos from which some blastomeres had been lost following cryopreservation. In conclusion, although children have been delivered after transfer of partially damaged embryos, the aim of a cryopreservation programme must be to obtain fully intact embryos after thawing.

译文

在我们的中心,如果至少一半的初始卵裂球数量保持完整,则判定胚胎可以在冷冻保存中存活。因此,完全完整和部分受损的胚胎都被转移了。这项研究的目的是研究冷冻保存后部分受损的人类胚胎的生存能力。我们回顾性分析了冷冻保存后完全完整或失去一些卵裂球的胚胎的植入和体内发育。用促性腺激素释放激素 (GnRH) 激动剂Buserelin和人类更年期促性腺激素刺激后收集卵母细胞。在循环的第2天或第3天,使用以二甲基亚砜为冷冻保护剂的缓慢冷却程序,将其体积不超过20% 的多细胞胚胎冷冻。缓慢解冻后,在314胚胎移植程序中转移431完全完整的胚胎,在327这样的程序中转移488部分受损的胚胎。在转移完全完整的胚胎后获得的具有胎儿心跳的妊娠囊的百分比几乎是转移部分受损的胚胎后的三倍 (11.4对3.5%)。45名儿童 (每次胚胎移植的出生率10%) 在完全完整的胚胎移植后出生,14名在冷冻保存后丢失了一些卵裂球的胚胎移植后出生。总之,尽管儿童是在转移了部分受损的胚胎后分娩的,但冷冻保存计划的目的必须是在解冻后获得完整的胚胎。

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